苗药石吊兰及其组织培养物中五种次生代谢产物含量变化与品质评价  

Content Changes and Quality Evaluation of Five Secondary Metabolites inLysionotus pauciflorus Maxim.and Its Tissue Culture

作　　者：窦全丽[1] 张仁波[1] 韦若勋[1] 李涛涛 张安琴 DOU Quanli;ZHANG Renbo;WEI Ruoxun;LI Taotao;ZHANG Anqin(College of Biology and Agricultural Science and Technology,Zunyi Normal College,Zunyi,Guizhou 563006)

机构地区：[1]遵义师范学院生物与农业科技学院,贵州遵义563006

出　　处：《北方园艺》2023年第10期107-114,共8页Northern Horticulture

基　　金：遵义市科技局资助项目(遵市科合计[2018]11号);遵义师范学院博士基金资助项目(遵师BS[2018]17号)。

摘　　要：以石吊兰及其不同发育阶段的组织培养物为试材,采用高效液相色谱法测定野生材料和组织培养物中5种次生代谢产物含量,研究了石吊兰组织培养过程中主要次生代谢产物的含量变化及不同材料的品质,以期为石吊兰药用价值的开发应用提供参考依据。结果表明:野生石吊兰不同部位中5种次生代谢产物含量差异显著,叶和嫩茎中连翘酯苷B含量最高,木质茎和根状茎中毛蕊花糖苷含量最高;石吊兰组织培养过程中次生代谢产物含量总体呈先升后降,以大车前苷、连翘酯苷B和木通苯乙醇苷B含量变化最明显,不同培养阶段材料中的次生代谢产物含量有显著差异;石吊兰及其组织培养前期的培养物中苯乙醇苷类成分含量丰富,可作为连翘酯苷B、大车前苷、木通苯乙醇苷B的原料来源,具有利用生物技术生产主要次生代谢产物的潜力。The contents of five secondary metabolites in wild materials and tissue cultures of Lysionotus pauciflorus Maxim.in different development stages were analyzed and evaluated using high performance liquid chromatography.The contents and quality of secondary metabolites in L.pauciflorus Maxim.and its tissue cultures were studied,in order to provide reference for development and utilization of L.pauciflorus Maxim..The results showed that the contents of five secondary metabolites in different parts were significantly different.The contents of forsythin B in leaves and tender stems were the highest,and the acteoside contents in woody stems and rhizomes were the highest;the content of secondary metabolites in the tissue culture process was generally increased first and then decreased.The content changes of plantain,forsythin B and xylitol B were the most obvious.The content of secondary metabolites in different culture stages was significantly different.In conclusion,the contents of phenylethanolic glycosides in L.pauciflorus Maxim.and its cultures at the early stage of tissue culture are relatively high.It can be used as the raw material source of forsythin B,plantain and xylitol glycoside B,and has the potential to produce major secondary metabolites by biotechnology.

关 键 词：石吊兰 野生材料 组织培养物 次生代谢产物 品质 

分 类 号：S567.9[农业科学—中草药栽培]