基于响应面法大球盖菇原生质体制备条件优化  

Optimization of Protoplast Formation of Stropharia rugosoannulate byResponse Surface Method

作　　者：吕锐玲 徐思琪 秦丽浩 崔高琴 郑永良 LYU Ruiling;XU Siqi;QIN Lihao;CUI Gaoqin;ZHENG Yongliang(College of Biology and Agricultural Resources,Huanggang Normal University/Hubei Key Laboratory for the Improvement of Economic Forest Germplasm and Comprehensive Utilization of Resources/Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains,Huanggang,Hubei 438000)

机构地区：[1]黄冈师范学院生物与农业资源学院,经济林木种质改良与资源综合利用湖北省重点实验室,大别山特色资源开发湖北省协同创新中心,湖北黄冈438000

出　　处：《北方园艺》2023年第10期115-120,共6页Northern Horticulture

基　　金：湖北省重点研发计划资助项目(2020BBB091);经济林木种质改良与资源综合利用湖北省重点实验室开放课题资助项目(2021ZD09);黄冈师范学院博士科研启动基金资助项目(2042020014);大学生创新创业训练计划资助项目(2022dc072)。

摘　　要：以大球盖菇菌丝体为试材,在单因素试验基础上,以原生质体产量为响应值,采用响应面方法优化其制备条件,研究了大球盖菇原生质体制备最佳条件,以期为后期大球盖菇菌种遗传改良提供参考依据。结果表明:经响应面试验优化后,获得大球盖菇原生质体的最佳制备条件为酶解时间2.5 h、酶解温度32.2℃、菌龄3.8 d,此时获得大球盖菇原生质体产量最大,为35.8×10^(6)CFU·mL^(-1),与理论产量(35.15×10^(6)CFU·mL^(-1))偏差较小。因此,基于响应面法确定的最佳制备条件可获得较高的原生质体产量。The mycelium of Stropharia rugosoannulate was used as the research material.The reaction conditions were optimized by response surface method based on the single factor test with protoplast yield as response value,in order to provide reference for genetic improvement of S.rugosoannulatain the later stage.The results showed that after the optimization of response surface method,the preparation conditions for the protoplasts were obtained as follows,enzymolysis time was 2.5hours,enzymolysis temperature was 32.2℃,and the mycelial age was 3.8days,the maximum protoplast yield was 35.8×10^(6) CFU·mL^(-1),Compared with the theoretical yield(35.15×10^(6) CFU·mL^(-1)),the deviation was small.Therefor,the optimum preparation conditions based on response surface method can obtain higher protoplast yield.

关 键 词：大球盖菇 原生质体 响应面法 

分 类 号：S646[农业科学—蔬菜学]