海洋来源真菌Eutypella sp. F0219细胞松弛素类代谢产物及其抗肿瘤活性  

Cytochalasins Isolated from the Marine-derived Fungus Eutypella sp.F0219 and Their Anti-tumor Activities

作　　者：苏为政 徐欣艺 欧婷婷 左青乐 马俊源 黎婉珊 SU Weizheng;XU Xinyi;OU Tingting;ZUO Qingle;MA Junyuan;LI Wanshan(Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education/College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158,China;Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province,Haikou 571158,China)

机构地区：[1]海南师范大学化学与化工学院/热带药用资源化学教育部重点实验室,海南海口571158 [2]海南省热带药用植物化学重点实验室,海南海口571158

出　　处：《海南师范大学学报（自然科学版）》2023年第2期143-149,共7页Journal of Hainan Normal University（Natural Science）

基　　金：海南省自然科学基金项目(221QN229);海南省重大科技计划项目(ZDKJ202008);大学生创新创业训练项目(202111658007,202211658019);海南省院士创新平台科研专项资金项目(YSPTZX202030)。

摘　　要：综合运用正、反相硅胶柱层析、薄层层析以及半制备HPLC等多种色谱方法对海洋真菌Eutypella sp.F0219的乙酸乙酯提取物进行系统分离纯化,获得8个细胞松弛素类化合物(1~8)。经一维核磁共振波谱数据、质谱以及X-射线单晶衍射方法等确定这些化合物分别为phenochalasin A (1),scoparasin D (2),phenochalasin B (3),cytochalasin Z26(4),cytochalasin Z24(5),[12]-cytochalasin (6),cytochalasin Z25(7)和cytochalasin Z27(8)。通过CCK-8实验评价上述化合物在非小细胞肺癌NCI-H1299细胞的增殖影响,筛选结果表明化合物2表现出中等的抗肿瘤活性,IC50值为(5.1±0.25)μmol/L。本研究首次报道了化合物phenochalasin A (1)的X-射线单晶结构。Eight cytochalasins were isolated from the ethyl acetate extract of the marine-derived fungus Eutypella sp.F0219 by applying silica gel and C18 revised-phase column chromatography,TLC,and semi-preparative HPLC methods.On the basis of 1D-NMR,ESI-MS,and single-crystal X-ray diffraction crystallography,the structures of these compounds were elucidated as phenochalasin A(1),scoparasin D(2),phenochalasin B(3),cytochalasin Z26(4),cytochalasin Z24(5),[12]-cytochalasin(6),cytochalasin Z25(7),cytochalasin Z27(8),respectively.These compounds were evaluated for cytotoxic activities against NCI-H1299 cell line by the assay of CCK-8.Compound 2 exhibited moderate cytotoxicity with IC50 value of(5.1±0.25)μmol/L.In addition,this is the first report of the X-ray structure of phenochalasin A(1).

关 键 词：海洋真菌 弯孢聚壳属 细胞松弛素 结构鉴定 抗肿瘤活性 

分 类 号：R281.4[医药卫生—中药学]