铁冬青-单面针浸膏中紫丁香苷、长梗冬青苷、氯化两面针碱、白鲜碱含量测定  

Determination of Syringin,Pedunculoside,Nitidine Chloride and Dictamnine in Ilex Rotunda Thunb and Zanthoxylum Dissitum Hemsl Extract

作　　者：彭佳欣 谭洋[1,2] 周喆 胡诗安 杨学斌 李玲 袁园[1,2] PENG Jiaxin;TAN Yang;ZHOU Zhe;HU Shian;YANG Xuebin;LI Ling;YUAN Yuan(School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Provincial Key Laboratory of Modernization of Traditional Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]湖南省普通高等学校中药现代化研究重点实验室,湖南长沙410208

出　　处：《海南师范大学学报（自然科学版）》2023年第2期183-191,共9页Journal of Hainan Normal University（Natural Science）

基　　金：湖南省自然科学基金项目(2020JJ5429)。

摘　　要：开展了HPLC法测定铁冬青-单面针及其浸膏中紫丁香苷、长梗冬青苷、氯化两面针碱、白鲜碱含量的方法学研究。确定了铁冬青的最优测定条件(流动相为乙腈-水;检测波长210 nm;流速1.0 mL/min;柱温30℃;进样量10μL)、单面针的最优测定条件(流动相为甲醇-0.1%甲酸水;检测波长272 nm;流速1.0 mL/min;柱温30℃;进样量10μL)、铁冬青-单面针浸膏的最优测定条件(流动相为乙腈-0.4%磷酸水;长梗冬青苷、白鲜碱检测波长为210 nm;紫丁香苷、氯化两面针碱检测波长为272 nm;流速1.0 mL/min;柱温30℃;进样量5μL)。在上述条件下,开展了HPLC测定法的线性范围、精密度、稳定性、重复性、回收率等实验,结果表明HPLC法可以较好地用于测定铁冬青-单面针及浸膏中紫丁香苷、长梗冬青苷、氯化两面针碱、白鲜碱的含量。HPLC was used to determine the contents of syringin,pedunculoside,nitidine chloride and Dictamnine in Ilex Rotunda Thunb and Zanthoxylum Dissitum Hemsl and Its Extract.The optimal determination conditions of Ilex Rotunda Thunb were determined(mobile phase:acetonitrile-water;detection wavelength 210 nm;flow rate 1.0 ml/min;column temperature was 30℃,sample size was 10μL).The optimal determination conditions of Zanthoxylum Dissitum Hemsl(mobile phase:methanol-0.1%formic acid water;Detection wavelength 272 nm;Flow rate 1.0 ml/min;column temperature 30℃;sample size 10μL),and the optimal determination conditions of Ilex Rotunda Thunb and Zanthoxylum Dissitum Hemsl Decoction(mobile phase:acetonitrile-0.4%phosphoric acid water,the detection wavelength of pedunculoside and dictamnine was 210 nm,and the detection wavelength of syringin and nitidine chloride was 272 nm.The flow rate is 1.0 mL/min.Column temperature 30℃;injection volume 5μL)were determined.Under the above conditions,the linear range,precision,stability,repeatability and recovery rate of the HPLC method were tested.The results showed that the HPLC method developed in this paper was good for the content determination of syringin,pedunculoside,nitidine chloride and dictamnine in Ilex Rotunda Thunb and Zanthoxylum Dissitum Hemsl and Its decoction.

关 键 词：铁冬青 单面针 含量测定 HPLC 

分 类 号：R284.2[医药卫生—中药学]