软坚散结方调控小鼠HIF-1α糖酵解分子转导抑制肝肿瘤细胞增殖机制研究  

Mechanism study on Ruanjian Sanjie prescription inhibit proliferation of liver cancer cells through regulating HIF-1αpathway

作　　者：陈嵘 李淑敏 李旭华 王振 杨飞 李兰青 杨文治[2] 万伟萍[2] 张志云[2] 张荣平 石西南 CHEN Rong;LI Shumin;LI Xuhua;WANG Zhen;YANG Fei;LI Lanqing;YANG Wenzhi;WAN Weiping;ZHANG Zhiyun;ZHANG Rongping;SHI Xinan(Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南中医药大学,云南昆明650500 [2]昆明市中医医院,云南昆明650032

出　　处：《陕西中医》2023年第7期839-842,848,共5页Shaanxi Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金地区基金资助项目(82060862);云南省科技厅-云南中医药大学联合项目[202101AZ070001-003/174/250/251,202101AG070-009/029,2019FF002(-050)/(-040),202001AZ070001-066/069];云南省科技人才和平台计划项目(202105AG070012);植物化学与西部植物资源持续利用国家重点实验室开放课题(P2022-KF04)。

摘　　要：目的:研究软坚散结方调控小鼠HIF-1α糖酵解分子通路抑制肝肿瘤细胞增殖作用。方法:肝癌移植瘤模型小鼠经软坚散结方治疗后,进行瘤组织mRNA筛查,比较组间基因表达及蛋白分子功能差异。结果:软坚散结方干预肝癌移植瘤模型小鼠后,软坚散结组瘤体明显小于对照组。瘤体组织mRNA筛查发现,软坚散结组HIF-1α表达高于对照组(P<0.05)。瘤体组织TUNEL实验表明,软坚散结组肿瘤细胞凋亡高于对照组,且瘤组织蛋白表达HIF-1α、Akt3、HK2、LDHA、GLUT1蛋白表达均低于对照组,而Cleaved Caspase-3高于对照组,差异有统计学意义(均P<0.05)。结论:软坚散结方通过调控小鼠HIF-1α糖酵解通路HK2、LDHA、GLUT1等蛋白水平,发挥抑制肝肿瘤细胞增生作用。Objective:To study Ruanjian Sanjie prescription impacting proliferation of liver cancer cells through regulating HIF-1αpathway in mice.Methods:After treatment with Ruanjian Sanjie prescription,mRNA screening of tumor tissue was performed to compare differences in gene expression and protein molecular function between the two groups.Results:After the intervention of Ruanjian Sanjie prescription in liver cancer transplantation model mice,the tumor size of Ruanjian Sanjie group was significantly smaller than that in the control group.mRNA screening of tumor tissue showed that HIF-1αexpression in Ruanjian Sanjie group was higher than that in the control group(P<0.05).TUNEL test of tumor tissue showed that the apoptosis of tumor cells in the Ruanjian Sanjie group was higher than that in the control group,and the expression of HIF-1α,Akt3,HK2,LDHA,GLUT1 in tumor tissue lower than those in the control group,while the Cleaved Caspase-3 was higher than that in the control group,difference statistical significance(all P<0.05).Conclusion:Ruanjian Sanjie prescription can inhibit proliferation of hepatocellular carcinoma cells by regulating levels of HK2,LDHA,GLUT1 and other proteins in mouse HIF-1αglycolysis pathway.

关 键 词：肝癌 软坚散结方 活血祛瘀 缺氧诱导因子 糖酵解 细胞增殖 

分 类 号：R273[医药卫生—中西医结合]