急性髓系白血病患者独立生长因子1B表达与各亚型诊断及临床治疗结局的关系  

Relationship between GFI1B expression in AML patients and diagnosis,clinical treatment outcomes of each subtype

作　　者：张志敏 刘鑫艳 张永梅 潘志兰 林凤茹[3] 赵晓君 ZHANG Zhimin;LIU Xinyan;ZHANG Yongmei;PAN Zhilan;LIN Fengru;ZHAO Xiaojun(Department of Hematology,Shijiazhuang People’s Hospital,Shijiazhuang 050000,China;不详)

机构地区：[1]石家庄市人民医院血液内科,河北石家庄050000 [2]石家庄市中医院内科,河北石家庄050011 [3]河北医科大学第二医院血液内科,河北石家庄050004

出　　处：《陕西医学杂志》2023年第7期850-854,共5页Shaanxi Medical Journal

基　　金：河北省医学科学研究计划项目(20210534,20160803)。

摘　　要：目的:探讨急性髓系白血病(AML)患者独立生长因子1B(GFI1B)的表达与各亚型诊断及临床治疗结局的关系。方法:选取AML患者60例(AML组)、急性淋巴细胞白血病患者(ALL组)26例、慢性粒细胞白血病患者(CML组)18例、骨髓增生异常综合征患者(MDS组)24例,健康志愿者(对照组)10例。采用定量聚合酶链反应(RQ-PCR)法检测各组患者GFI1B基因表达水平;并将AML患者根据法国(Franch)、美国(American)和英国(Britain)制定的(FAB)分型标准分型,对患者治疗结局进行分层比较。结果:AML组患者的GFI1B mRNA表达水平显著高于ALL组、CML组、MDS组和对照组,差异具有统计学意义(均P<0.05);CML组患者的GFI1B mRNA表达水平高于ALL组、MDS组和对照组,差异具有统计学意义(均P<0.05);ALL组、MDS组患者的GFI1B mRNA表达水平高于对照组,差异具有统计学意义(均P<0.05);M 7型AML组患者的GFI1B mRNA表达水平显著高于其他分型的AML患者,差异具有统计学意义(均P<0.05);M 6型AML组患者的GFI1B mRNA表达水平显著高于其他分型(M 1-M 5)的AML患者,差异具有统计学意义(均P<0.05);M 4型AML组患者的GFI1B mRNA表达水平显著高于其他分型(M 1、M 2、M 3、M 5)的AML患者,差异具有统计学意义(均P<0.05);60例接受化疗的AML患者中,完全缓解(CR)患者32例、部分缓解(PR)患者16例、未缓解(NR)患者12例;CR+PR组患者治疗前及治疗后的GFI1B mRNA表达水平均显著低于NR组患者,差异具有统计学意义(均P<0.05)。结论:AML患者的GFI1B mRNA表达水平显著高于其他类型白血病患者,其中M 7、M 6型的GFI1B mRNA表达水平最高;GFI1B mRNA表达水平与AML患者化疗效果有一定的关系。Objective:To investigate the relationship between the expression of growth factor-independent 1B(GFI1B)in patients with acute myeloid leukemia(AML)and the diagnosis,clinical treatment outcomes of each subtype.Methods:A total of 60 patients with AML(AML group),26 patients with acute lymphoblastic leukemia(ALL group),18 patients with chronic myeloid leukemia(CML group),24 patients with myelodysplastic syndrome(MDS group)and 10 healthy volunteers(control group)were selected.RQ-PCR was used to detect the expression of GFI1B gene in each group.The AML patients were classified according to FAB classification criteria,and the treatment outcomes of the patients were stratified and compared.Results:The mRNA expression level of GFI1B in AML group was significantly higher than that in ALL group,CML group,MDS group and control group(all P<0.05).The expression level of GFI1B mRNA in CML group was higher than that in ALL group,MDS group and control group(all P<0.05).The expression level of GFI1B mRNA in ALL group and MDS group was higher than that in control group(all P<0.05).The expression level of GFI1B mRNA in M 7 AML group was significantly higher than that in other types of AML patients(all P<0.05).The mRNA expression level of GFI1B in M 6 AML group was significantly higher than that in M 1 to M 5 AML(all P<0.05).The expression level of GFI1B mRNA in M 4 AML group was significantly higher than that in M 1,M 2,M 3,M 5 AML(all P<0.05).Among 60 AML patients who received chemotherapy,32 achieved CR,16 achieved PR and 12 achieved NR.The mRNA expression levels of GFI1B in CR+PR group before and after treatment were significantly lower than those in NR group(all P<0.05).Conclusion:The expression level of GFI1B mRNA in AML patients was significantly higher than that in patients with other types of leukemia,among which M 7 and M 6 types had the highest GFI1B mRNA expression levels.The expression level of GFI1B mRNA had a certain relationship with the chemotherapy effect of AML patients.

关 键 词：急性髓系白血病 独立生长因子1B 慢性粒细胞白血病 骨髓增生异常综合征 定量聚合酶链反应 

分 类 号：R552[医药卫生—血液循环系统疾病]