参附注射液基于TLRs信号通路对慢性心力衰竭大鼠模型保护作用的机制研究  

Research on the Protective Mechanism of Shenfu Injection on Chronic Heart Failure Rat Model Based on Toll-like Signal Pathway

作　　者：杨柳[1] 叶东 胡耀[1] 曾圣强 徐文艳 王洪[1] YANG Liu;YE Dong;HU Yao;ZENG Shengqiang;XU Wenyan;WANG Hong(Department of Cardiology,Jiangxi Provincial People’s Hospital/The First AffiliatedHospital of Nanchang Medical College,Jiangxi Province,Nanchang 330006,China;Department of Cardiology,Yongxiu County People’s Hospital,Jiangxi Province,Jiujiang330300,China;Graduate School,Nanchang University,Jiangxi Province,Nanchang 330000)

机构地区：[1]江西省人民医院/南昌医学院第一附属医院心内科,江西南昌330006 [2]江西省九江市永修县人民医院心内科,江西九江330300 [3]南昌大学医学院,江西南昌330000

出　　处：《中国中医药现代远程教育》2023年第13期134-137,共4页Chinese Medicine Modern Distance Education of China

基　　金：江西省中医药科技计划项目【No.2020B0316】;江西省科技厅重点研发计划项目【No.20161BBG70116】;南昌医学院第一附属医院江西省人民医院博士启动基金【No.19-236号】。

摘　　要：目的探讨参附注射液对慢性心力衰竭大鼠模型的保护作用以及Toll-like受体(TLRs)信号通路在其中的作用。方法构建大鼠心衰模型,并随机将40只大鼠分为对照组、参附注射液低剂量组、参附注射液高剂量组和模型组。使用心肌细胞分别进行了实时定量PCR和Westernblot分析,检测Toll-like受体4(TLR4)、髓样分化因子(MyD88)、肿瘤坏死因子受体相关分子6(TRAF6)的mRNA和蛋白表达。结果参附注射液高剂量组中,TLR4、MyD88和TRAF6的mRNA表达水平明显下降(低剂量组降低不显著)。参附注射液高剂量组中,TLR4、MyD88、TRAF6蛋白表达水平显著下降(低剂量组降低不显著)。结论参附注射液在慢性心力衰竭大鼠模型中有保护心肌作用,它可能是通过抑制炎症反应以及心肌纤维化来实现的,其机制与TLRs信号路径有关。Objective To investigate the protective effect of Shenfu injection on chronic heart failure rat model and the role of Toll-like receptor(TLRs)signaling pathway.Methods A rat heart failure model was established,and 40 rats were randomly divided into control group,Shenfu injection low-dose group,Shenfu injection high-dose group and model group.The mRNA and protein expressions of Toll-like receptor 4(TLR4),myeloid differentiation factor(MyD88)and tumor necrosis factor receptor-associated molecule 6(TRAF6)were detected by real-time quantitative PCR and Western blot analysis of cardiomyocytes,respectively.Results The mRNA expression levels of TLR4,MyD88 and TRAF6 in the high-dose group of Shenfu injection were significantly decreased(the decrease was not significant in the low-dose group).The protein expression levels of TLR4,MyD88 and TRAF6 in the high-dose group of Shenfu Injection were significantly decreased(the decrease was not significant in the low-dose group).Conclusion Shenfu injection can protect myocardium in rats with chronic heart failure,which may be achieved by inhibiting inflammatory response and myocardial fibrosis,and its mechanism is related to TLRs signaling pathway.

关 键 词：参附注射液 慢性心力衰竭 大鼠模型 TLRs信号通路 实验研究 

分 类 号：R285.5[医药卫生—中药学]