胶体金免疫层析法定量检测烟叶中霜霉威残留  

Quantitative detection of propamocarb residues in tobacco leaf by colloidal gold immunochromatography

作　　者：王春琼 陈丹 罗旭轲 郭建 杨飞[5] 孙浩巍 张轲 蔡洁云 张晓伟 李晓芳 王兆芹 WANG Chunqiong;CHEN Dan;LUO Xuke;GUO Jian;YANG Fei;SUN Haowei;ZHANG Ke;CAI Jieyun;ZHANG Xiaowei;LI Xiaofang;WANG Zhaoqin(Yunnan Tobacco Quality Supervision and Test Station,Kunming 650106,China;Beijing Kwinbon Technology Co.,Ltd.,Beijing 102206,China;Beijing Engineering Research Centre of Food Safety Immunodetection,Beijing 102206,China;Honghe Prefecture Branch of Yunnan Tobacco Company,Mile 652300,China;China National Tobacco Quality Supervision&Test Center,Zhengzhou 450001,China)

机构地区：[1]云南省烟草质量监督检测站,云南昆明650106 [2]北京勤邦科技股份有限公司,北京102206 [3]北京市食品安全免疫快速检测工程技术研究中心,北京102206 [4]云南省烟草公司红河州公司,云南652300 [5]国家烟草质量监督检验中心,河南郑州450001

出　　处：《中国烟草学报》2023年第3期57-65,共9页Acta Tabacaria Sinica

基　　金：中国烟草总公司云南省公司科技项目“烟叶农残及病害快速检测技术的研发与应用”(2021530000241021)。

摘　　要：【背景和目的】利用胶体金免疫层析技术研制霜霉威定量检测试纸条,并探究其在烟叶样品中的应用。【方法】将霜霉威单克隆抗体标记在胶体金颗粒上制备金标抗体,优化试纸条反应材料,构建霜霉威免疫层析定量试纸条,验证该试纸条的检出限、定量限、准确性和特异性。【结果】(1)所研制的霜霉威定量试纸条的检测范围为1.25~20 mg/kg,检出限和定量限分别为1.187 mg/kg和3.147 mg/kg;加标回收率84.7%~116.7%,相对标准偏差5.3%~10.9%。(2)可特异性识别霜霉威,对烟草中常用杀菌剂无交叉反应。(3)试纸条检测结果与高效液相色谱-串联质谱法检测结果的相对误差在4.3%~20.1%,R^(2)=0.9898。【结论】该试纸条具有操作方便、检测时间短、准确性高、特异性强的优点,可作为霜霉威残留现场检测的有效手段。[Objective]This study aims to develop the strip for quantitative detection of propamocarb using colloidal gold immunochromatographic technique,and explore its application in tobacco samples.[Methods]The anti-propamocarb monoclonal antibody were labeled on colloidal gold to prepare gold labeled antibody.Reactive materials were optimized to develop immuno-colloidal gold chromatographic strip for quantitative detection of propamocarb.The limit of detection(LOD),quantitation(LOQ),the accuracy and specificity of the test strip were verified.[Results]1)The detection range of the developed immune-colloidal gold chromatographic test strip was 1.25-20 mg/kg,and the LOD and LOQ for propamocarb were 1.187 mg/kg and 3.147 mg/kg,respectively.The recoveries were 84.7%-116.7%with the relative standard deviation ranging from 5.3%to 10.9%.2)The developed test trip was able to identify the propamocarb specifically without cross-reacting with the fungicides commonly used in tobacco.3)The relative error of the results between the test strip and the high performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS)was 4.3%-20.1%,and the linear correlation coefficient R^(2)=0.9898.[Conclusion]The developed test strip has the advantages of convenient operation,short detection time,high accuracy and strong specificity,which can be used as an effective method for on-site detection of propamocarb residues.

关 键 词：霜霉威 烟叶 胶体金免疫层析技术 定量检测 

分 类 号：TS47[农业科学—烟草工业]