电针对后循环缺血性眩晕模型大鼠可溶性SSAO/VAP-1释放的影响  

Effect of EA on Release of Soluble SSAO/VAP-1 in Rats with PCIV

作　　者：李华 李国徽[2] 王强[1] 郭婕 张改月 LI Hua;LI Guohui;WANG Qiang;GUO Jie;ZHANG Gaiyue(Shaanxi University of Chinese Medicine,Xianyang 712000,China;Yinchuan Hospital of TCM Affiliated to Ningxia Medical University,Yinchuan 750001,China)

机构地区：[1]陕西中医药大学,陕西咸阳712000 [2]宁夏医科大学附属银川中医医院,宁夏银川750001

出　　处：《针灸临床杂志》2023年第6期80-87,共8页Journal of Clinical Acupuncture and Moxibustion

基　　金：银川市科技创新重大重点专项“眩晕病多学科临床医学研究中心开发应用”,编号:2021-SF-003;全国中医药创新骨干人才培训项目,编号:宁中医发﹝2019﹞12号。

摘　　要：目的:观察电针干预对后循环缺血性眩晕(PCIV)模型大鼠可溶性SSAO/VAP-1释放及其调节的黏附分子和相关炎症因子表达的影响,探讨电针改善PCIV模型大鼠运动功能的部分作用机制。方法:采用数字表法将大鼠随机分为15只假手术组,其余30只大鼠采用双侧颈总动脉结扎法建立后循环缺血性眩晕大鼠模型,将造模完成后的大鼠随机分为模型组、电针组,每组15只。于造模成功后开始电针干预“风池”“悬钟”,频率2 Hz,电流强度1 mA,20 min/次,1次/d,共干预10 d。干预当天开始记录大鼠的体征变化。分别在造模前、造模后和干预后对各组大鼠进行平衡试验,采用眩晕测试测定大鼠跳台逃避潜伏时间。治疗结束后苏木精-伊红染色观察大鼠脑皮质病理学变化;采用免疫印迹法检测大鼠SSAO/VAP-1及其粘附因子和炎症因子的表达水平;免疫荧光观察大鼠脑皮质IκB-α阳性细胞的表达。结果:造模结束后,模型组大鼠毛色灰暗,精神不振,反应迟钝,电针干预后大鼠机敏灵活,体质量增加(P<0.05),平衡试验阳性率降低(P<0.01)。与模型组相比,电针组大鼠跳台逃避时间缩短(P<0.01);脑皮质形态、神经元细胞结构在一定程度上得以修复;血样中可溶性SSAO/VAP-1表达水平下降(P<0.01);脑皮质中SSAO/VAP-1调节的粘附分子E-selectin、VCAM-1及炎症因子COX-2、SOD-1表达水平下降(P<0.05或P<0.01);IκB-α阳性表达升高(P<0.05)。结论:电针可改善PCIV模型大鼠眩晕症状及神经元形态结构与功能,机制可能与其抑制可溶性SSAO/VAP-1释放从而减少炎症细胞的循环募集、减弱炎症对血管的损伤、促进脑组织血循环通畅和前庭血流正常有关。Objective:To observe the effect of electro-acupuncture(EA)intervention on the release of soluble SSAO/VAP-1 and the expression of adhesion molecules and related inflammatory factors in treatment of posterior circulation ischemic vertigo(PCIV),and to explore the mechanism of EA on improving motor function in PCIV model rats.Methods:The rats were randomly divided into the sham group,the model group and the EA group,with 15 rats in each group.The rat model of PCIV was established by bilateral common carotid artery ligation.After successful modeling,EA intervention(2 Hz,1 mA,20 min/time,1 time/d)was applied to Fengchi(GB20)and Xuanzhong(GB39)for 10 days.Changes in physical signs of rats were recorded from the day of intervention.Balance test was carried out before modeling,after modeling and after intervention;the escape latency time was determined by vertigo test.Pathogenic changes in cerebral cortex were observed by HE staining;the expressions of SSAO/VAP-1 and its adhesion factors and inflammatory factors were detected by Western blot;the expression of IκB-αpositive cells in cerebral cortex was observed by immunofluorescence.Results:The rats in the model group showed dark gray hair,low activity,slow response;after EA intervention,the rats were flexible and responsive,and they gained weight(P<0.05),and the positive rate of balance test decreased(P<0.01).Compared with those in the model group,the escape latency time was shortened(P<0.01),cerebral cortex morphology and neuronal cell structure were repaired to some extent,the expression of soluble SSAO/VAP-1 was decreased in blood sample(P<0.01);SSAO/VAP-1-regulated adhesion molecules E-selectin,VCAM-1 and inflammatory factors of COX-2 and SOD-1 were decreased in cerebral cortex(P<0.05,P<0.01),and IκB-αpositive expression was increased in the EA group(P<0.05).Conclusion:EA can improve the symptoms of vertigo and the morphological structure and function of neurons in PCIV model rats.The mechanism may be related to inhibiting the release of soluble SSAO/VAP-1 to red

关 键 词：后循环缺血性眩晕 电针 SSAO/VAP-1 粘附分子 超氧化物歧化酶 IΚB-Α 

分 类 号：R246.6[医药卫生—针灸推拿学]