具有荧光开关特性的喹啉衍生物用于高效选择性标记细胞脂滴  

Quinoline Derivatives with Fluorescent Switching Properties for Efficient Selective Labeling of Cellular Lipid Droplets

作　　者：赵力 周日 刘冠男 彭桂衫 王晨光 贾晓腾 卢革宇[1] Zhao Li;Zhou Ri;Liu Guannan;Peng Guishan;Wang Chenguang;Jia Xiaoteng;Lu Geyu(State Key Laboratory of Integrated Optoelectronics,School of Electronic Science and Engineering,Jilin University,Changchun 130012,Jilin,China)

机构地区：[1]吉林大学电子科学与工程学院集成光电子学国家重点实验室,吉林长春130012

出　　处：《中国激光》2023年第9期9-15,共7页Chinese Journal of Lasers

基　　金：国家自然基金(62075079)。

摘　　要：脂滴是一种重要的细胞器,与细胞中多种生理活动密切相关。为了观察脂滴并研究其多种多样的功能,共聚焦荧光成像技术是最有力的工具之一。然而,细胞脂滴荧光成像所需的具有高荧光亮度和高标记选择性的脂滴荧光探针却十分有限,这严重限制了脂滴的深入研究。研制了一种具有荧光开关特性的喹啉衍生物脂滴荧光探针(Lipi-QL)。该探针因其敏感的极性淬灭荧光特性,展现出了很高的脂滴标记选择性。Lipi-QL能靶向脂滴是因为其脂溶性,进入脂滴后,荧光增强是脂滴内的较低极性引起的。给受体型分子结构还赋予了该探针高的荧光亮度以及大的斯托克斯位移。将该探针用于细胞脂滴共聚焦荧光成像,在不同浓度下都实现了显著优于脂滴商用探针BODIPY 493/503的标记选择性。此外,使用该荧光探针实现了固定细胞的三维共聚焦成像和活细胞的四色共聚焦成像。该荧光探针的研制一方面为脂滴生理功能的研究提供了强有力的工具,另一方面也为新型高标记选择性荧光探针的设计提供了新的思路。Objective Lipid droplets are important organelles closely associated with various cellular physiological activities.Confocal fluorescence imaging is a powerful tool for observing lipid droplets and studying their diverse functions.However,lipid droplet fluorescent probes with the high fluorescence intensity and labeling selectivity required for cellular lipid droplet fluorescence imaging are limited,severely limiting the in-depth study of lipid droplets.In this study,we develop Lipi-QL,a quinoline-derivative lipid droplet fluorescent probe with fluorescence-switching properties.Methods The probe exhibits high selectivity for lipid droplet labeling owing to its sensitive polar quenching fluorescence properties.The donor-type molecular structure also confers high fluorescence intensity and large Stokes shifts on the probe.When using this probe for confocal fluorescence imaging of cellular lipid droplets,significantly better labeling selectivity is achieved at varying concentrations than when using the commercial BODIPY 493/503 lipid droplet probe.Additionally,three-dimensional confocal imaging of fixed cells and four-color confocal imaging of live cells are performed using this fluorescent probe.The development of this probe provides a powerful tool for studying the physiological functions of lipid droplets and provides a new idea for the design of new highly labeled selective fluorescent probes.Results and Discussions As shown in Fig.1(c),the probe exhibits highly efficient fluorescence emission when the water volume fraction is 0,indicating that it can exhibit high fluorescence intensity within lipid droplets.When the water volume fraction gradually increases,the probe exhibits extremely sensitive fluorescence quenching properties:quenching most of the fluorescence emission when the water volume fraction is only 1%.When the water volume fraction increases to 20%,the probe s emission is almost completely quenched,and the fluorescence signal disappears.This indicates that even if a small portion of the probe enters

关 键 词：医用光学 荧光开关特性 荧光成像 荧光探针 脂滴 

分 类 号：Q28[生物学—细胞生物学]