细菌自体荧光检测装置的研究与设计  

Research and Design of Bacterial Autofluorescence Detection Device

作　　者：郑家运 王贻坤 倪敬书 张洋 黄尧 张元志 王全福 王霞 刘勇[2,3] 周金华 董美丽[2] ZHENG Jiayun;WANG Yikun;NI Jingshu;ZHANG Yang;HUANG Yao;ZHANG Yuanzhi;WANG Quanfu;WANG Xia;LUI Yong;ZHOU Jinhua;DONG Meili(School of Biomedical Engineering,Anhui Medical University,Hefei 230032,China;Anhui Provincial Engineering Laboratory for Medical Optical Diagnosis&Treatment Technology and Instrument,Anhui Provincial Engineering Technology Research Center for Biomedical Optical Instrument,Anhui Institute of Optics and Fine Mechanics,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,China;Wanjiang Center for Development of Emerging Industry Technology,Tongling 244000,China)

机构地区：[1]安徽医科大学生物医学工程学院,合肥230032 [2]中国科学院合肥物质科学研究院安徽光学精密机械研究所安徽省生物医学光学仪器工程技术研究中心,安徽省医用光学诊疗技术与装备工程实验室,合肥230031 [3]皖江新兴产业技术发展中心,铜陵244000

出　　处：《光子学报》2023年第6期294-303,共10页Acta Photonica Sinica

基　　金：安徽省科技重大专项(No.202103a07020008);中国科学院合肥物质科学研究院院长基金(Nos.YZJJZX202009,YZJJ2021GCH05);安徽省食品药品监督管理局科技创新项目(No.AHYJ-KJ-202114)。

摘　　要：细菌感染会造成伤口愈合延迟,增加患者和医疗卫生系统的负担,因此非常需要对伤口细菌进行及时检测和适当的处理。基于细菌自发荧光原理,设计了一种对伤口常见细菌快速检测成像的系统,可用于辅助识别伤口细菌负荷和判断感染情况,无需采样培养或染色鉴定。使用智能手机采集细菌荧光图像,以405 nm LED作为细菌激发光源,配合荧光图像信息提取和定量算法能够快速识别提取图像上的感兴趣区域并对荧光信号进行量化,定位细菌荧光信号区域。梯度浓度实验证实了荧光强度与细菌浓度高度相关,荧光强度与浓度之间存在线性关系,随着细菌浓度的降低,荧光信号强度呈下降趋势,系统最小检测限约为105 CFU/mL。与传统细菌检测方法相比,本装置具有操作简单、检测速度快、成本低等特点,为伤口细菌检测提供了一种新的手段。Excessive bacterial load will not only cause delayed wound healing and local or systemic inflammatory reaction,but also threaten the life safety of patients in severe cases.Therefore,a new method that can detect wound bacteria quickly and directly is needed.Traditional bacterial detection methods are mainly visual observation of clinical symptoms(including fever,redness and swelling,pus exudate,etc.)and microbial swab sampling,but these two methods are usually subjective and time-consuming.The results of the same clinical symptom observed by doctors with different experiences may be different,and in some cases the infection may not show obvious symptoms.Therefore,it is often subjective to judge the infection by observing the clinical symptoms with naked eyes and may cause misdiagnosis.Swab sampling is often plagued by false negative,and different sampling methods and selection of sampling areas will affect the results of sampling and culture.False negative areas or missed areas may contain a large number of bacteria.The missing of a large number of bacteria in these areas may lead to repeated wound healing,causing great pain to patients and great burden on the medical and health system.In addition,longer culture time is also a pain point for swab sampling,because it may cause inaccurate culture results and miss the best treatment opportunity.Relevant studies have found that bacteria can emit fluorescence by themselves under the excitation of 405 nm light source,without using contrast agents.The purpose of this study is to design a fluorescence detection imaging device based on the principle of spontaneous fluorescence of bacteria,which provides a fast and direct new method for wound bacteria detection.In this study,a bacterial fluorescence imaging system is developed.The light source module of the system consists of two LED with a central wavelength of 405 nm and LED driver modules.After calibration and parameter setting,the light source driver works in constant current mode to ensure stable operation of the ligh

关 键 词：细菌自体荧光成像 细菌检测 感染 图像处理 细菌量化 

分 类 号：TN29[电子电信—物理电子学]