2018年广东省广州市鸡源和猪源鼠伤寒沙门菌耐药性及分子特征分析  

Antibiotic resistance and molecular characteristics of Salmonella Typhimurium isolated from chicken and pork

作　　者：朱坤鹏 向莹 邱少富 杨海燕[1] 杜昕颖 Zhu Kunpeng;Xiang Ying;Qiu Shaofu;Yang Haiyan;Du Xinying(School of Public Health,Zhengzhou University,Zhengzhou 450001,Henan,China;The Centre of Disease Control and Prevention of Chinese People's Liberation Army,Beijing 100071,China)

机构地区：[1]郑州大学公共卫生学院,河南郑州450001 [2]解放军疾病预防控制中心,北京100071

出　　处：《疾病监测》2023年第5期567-573,共7页Disease Surveillance

基　　金：国家自然科学基金(No.82173580,No.82202538,No.81873968)。

摘　　要：目的调查2018年广州市鸡源和猪源鼠伤寒沙门菌的耐药性及分子特征,为合理使用抗生素和耐药菌防控提供依据。方法使用API 20E生化试验鉴定鼠伤寒沙门菌。使用CMV3AGNF药敏板进行药敏试验。利用Illumina Miseq测序平台进行全基因组测序,分析序列类型、抗生素耐药基因和点突变,以及构建系统发育树。结果广州市鸡源(15株)和猪源(21株)鼠伤寒沙门菌对氨苄青霉素、萘啶酸、四环素、氯霉素和磺胺异恶唑的耐药率≥60.00%,多重耐药率超过65.00%,以氨苄西林-萘啶酸-四环索-氯霉素-(甲氧苄氨嘧啶/磺胺甲恶唑-磺胺异恶唑)-庆大霉素耐药谱为主,且猪源菌耐药更为严重。猪源菌对tet(B)、aph(3'')-Ib和aph(6)-Id的携带率高于鸡源菌(P<0.05),携带更多的耐药基因(P<0.05)。GyrA点突变在鸡源菌和猪源菌的分布差异无统计学意义。系统发育及序列类型分析发现,鼠伤寒沙门菌共分为4个分支,鸡源菌更具遗传多态性,C4优势分支同时包含鸡源菌和猪源菌。结论鼠伤寒沙门菌耐药普遍,猪源菌的耐药则更为严重。畜牧业应合理使用抗生素,应加强鼠伤寒沙门菌的病原监测尤其是对猪肉食品的监测,防控耐药菌的传播。Objective To investigate the antibiotic resistance and molecular characteristics of Salmonella Typhimurium isolated from chicken and pork in Guangzhou,2018,and provide data support for the rational use of antibiotics and the prevention of drug-resistant pathogens.Methods The S.Typhimurium isolates were identified by using API 20E biochemical tests,and antibiotic susceptibility testing was performed by using Sensititre CMV3AGNF plates.The whole genome was sequenced by using Illumina Miseq platform to analyze sequence types(ST),antibiotic resistance genes(ARGs)and point mutations,and to construct phylogenetic tree.Results The antibiotic resistance rates of S.Typhimurium strains isolated from chicken(n=15)and pork(n=21)in Guangzhou to ampicillin,nalidixic acid,tetracycline,chloramphenicol,and sulfisoxazole were≥60.00%,and the multi-drug resistance(MDR)rates were more than 65.00%.The ampicillin-nalidixic acid-tetracyclinechloramphenicol-(trimethoprim/sulfamethoxazole-chloramphenicol)-gentamicin was the predominant antibiotic resistance pattern,and the drug resistance of S.Typhimurium isolated from pork was more serious.The carriage rates of tet(B),aph(3")-Ib,and aph(6)-Id were higher in pork isolates than in chicken isolates,and more ARGs were found in pork isolates.There was no difference in the distribution of gyrA point mutations between isolates from chicken and pork.Phylogenetic analysis and sequence type analysis showed that the S.Typhimurium were divided into four clades.The chicken isolates were more genetically polymorphic,and the C4 dominant clades contained both isolates from chicken and pork.Conclusion The antibiotic resistance of S.Typhimurium is common,and the antibiotic resistance of the pathogen from pork is more serious.Antibiotics should be used rationally in animal husbandry.It is necessary to further strengthen the S.Typhimurium surveillance,especially in pork food,for the prevention of resistant pathogens.

关 键 词：鼠伤寒沙门菌 鸡源 猪源 耐药率 耐药基因 

分 类 号：R211[医药卫生—中医学] R378