一株同时携带bla_(CTX-M-14)和bla_(CTX-M-15)基因的多重耐药宋内志贺菌耐药基因及质粒分析  

Resistance genes and plasmids of a multi-drug resistant Shigella sonnei strain carrying both bla_(CTX-M-14) and bla_(CTX-M-15) genes

作　　者：刘康康 李春晓 Liu Kangkang;Li Chunxiao(Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)

机构地区：[1]军事科学院军事医学研究院,北京100850

出　　处：《疾病监测》2023年第5期574-580,共7页Disease Surveillance

摘　　要：目的对1株同时耐头孢菌素和阿奇霉素的多重耐药宋内志贺菌进行全基因组测序分析,明确其携带的耐药基因和质粒特征。方法通过对2011年上海市的60株宋内志贺菌进行抗菌药物敏感性分析,筛选出1株同时耐头孢菌素和阿奇霉素的菌株Sh11sh529,使用二代和纳米孔测序技术获得其基因组序列,分析基因组的耐药基因、质粒、可移动遗传元件等。结果Sh11sh529基因组中检测到了11个耐药基因以及gyrA基因喹诺酮耐药决定区(QRDR)的突变位点gyrA-S83L,耐药基因包括2种CTX-M型超广谱β内酰胺酶(ESBL)基因bla_(CTX-M-14)和bla_(CTX-M-15),其中bla_(CTX-M-14)、mphA、aac(3)-IId、dfrA17、aadA5、sul1和qacEdelta基因由1个IncB/O/K/Z型质粒pSh11sh529-2携带,bla_(CTX-M-14)上游有1个ISEcp1插入子,mphA上游有1个IS26插入子。而bla_(CTX-M-15)由IncFII型质粒pSh11sh529-3携带,在其上游有1个ISEcp1插入子。该菌株表现出多重耐药性,对头孢菌素类、大环内酯类、氨基糖苷类、磺胺类、青霉素类等5类抗菌药物中的9种耐药。结论应当加强对多重耐药菌株以及同时携带多个类型ESBL菌株的监测和研究,以制定策略减少其流行和传播。Objective To analyze the whole genome sequencing of a multi-drug resistant Shigella sonnei strain resistant to both cephalosporin and azithromycin and identify the characteristics of its resistance genes and plasmids.Methods A cephalosporin and azithromycin resistant S.sonnei strain(Sh11sh529)was screened by analyzing the antibiotic susceptibility of 60 S.sonnei strains collected from Shanghai in 2011,and genome sequence of the strain was obtained by second-generation sequencing and nanopore sequencing technology.The drug resistance genes,plasmids and genetic mobile elements were analyzed.Results Eleven resistance genes and gyrA-S83L mutation sites in quinolone resistance determining region(QRDR)of gyrA gene were detected in genome of the Sh11sh529 strain.The resistance genes included two extended spectrumβ-lactamases(ESBLs)genes bla_(CTX-M-14) and bla_(CTX-M-15).The bla_(CTX-M-14),mphA,aac(3)-IId,dfrA17,aadA5,sul1 and qacEdelta genes were carried by an IncB/O/K/Z plasmid(pSh11sh529-2).There was an ISEcp1 insert in upstream of bla_(CTX-M-14),an IS26 insert in upstream of mphA.The bla_(CTX-M-15) gene was carried by an IncFII plasmid(pSh11sh529-3),and there was an ISEcp1 insert in upstream of bla_(CTX-M-15).The strain was resistant to 9 antibiotics of 5 classes,including cephalosporins,macrolides,aminoglycosides,sulfonamides,and penicillins.Conclusion It is necessary to strengthen the surveillance and research of multi-drug resistant strains and strains carrying multiple types of ESBL for the development of strategies to reduce the prevalence and spread of multi-drug resistant strains.

关 键 词：宋内志贺菌 多耐药菌株 超广谱β内酰胺酶 质粒 基因组测序 

分 类 号：R211[医药卫生—中医学] R378