Label-free homogeneous photoelectrochemical aptasensing of VEGF_(165)based on DNA-regulated peroxidase-mimetic activity of metal-organic-frameworks  

作　　者：Ting Hou Ningning Xu Xin Song Limin Yang Feng Li 

机构地区：[1]College of Chemistry and Pharmaceutical Sciences,Qingdao Agricultural University,Qingdao 266109,China

出　　处：《Chinese Chemical Letters》2023年第6期215-218,共4页中国化学快报（英文版）

基　　金：funded by the National Natural Science Foundation of China(Nos.22174083 and 22076090);Shandong Provincial Natural Science Foundation(No.ZR2020ZD37);Shandong Province Higher Educational Program for Young Innovation Talents。

摘　　要：The application of metal-organic frameworks(MOFs)nanozymes in biosensing has been extensively investigated,however,till now there is still no report on photoelectrochemical(PEC)sensing based on enzyme memetic properties of MOFs.To further expand the utilization of MOFs nanozymes in biosensing,we developed a label-free homogenous PEC aptasensor for the detection of VEGF_(165),an important cancer biomarker,based on the DNA-regulated peroxidase-mimetic activity of Fe-MIL-88,a type of MOFs.In this strategy,the peroxidase-mimetic property of MOFs is integrated with the label-free homogeneous PEC sensing approach,and highly sensitive detection of VEGF_(165)is obtained with a detection limit down to 33 fg/m L,superior or comparable to the previously reported values.Moreover,this approach displays outstanding specificity,and has been successfully used to detect VEGF_(165)added in diluted serum samples.As far as we know,it is the first example to employ the peroxidase-like activity of MOFs in PEC biosensing,which may find potential application in bioanalysis and early disease diagnosis.

关 键 词：Homogenous photoelectrochemistry Aptasensing Label free VEGF_(165) Fe-MIL-88 Peroxidase-like activity 

分 类 号：R318[医药卫生—生物医学工程] TP212[医药卫生—基础医学]