Advanced Pt hollow nanospheres/rubrene nanoleaves coupled with M-shaped DNA walker for ultrasensitive electrochemiluminescence bioassay  

作　　者：Yumeng Song Xiuli Tao Wenbin Liang Xia Zhong Ruo Yuan Ying Zhuo 

机构地区：[1]Key Laboratory of Luminescence Analysis and Molecular Sensing,Ministry of Education,College of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China

出　　处：《Chinese Chemical Letters》2023年第6期247-251,共5页中国化学快报（英文版）

基　　金：financially supported by the National Natural Science Foundation(NNSF)of China(No.22022408);the Chongqing Talents Personnel Support Program(No.NCQYC201905067);the Fundamental Research Funds for the Central Universities(No.XDJK2019TJ002)。

摘　　要：Herein,an intense electrochemiluminescence(ECL)was achieved based on Pt hollow nanospheres/rubrene nanoleaves(Pt HNSs/Rub NLs)without the addition of any coreactant,which was employed for ultrasensitive detection of carcinoembryonic antigen(CEA)coupled with an M-shaped DNA walker(M-DNA walker)as signal switch.Specifically,in comparison with platinum nanoparticles(Pt NPs),Pt HNSs revealed excellent catalytic performance and pore confinement-enhanced ECL,which could significantly amplify ECL intensity of Rub NLs/dissolved O_(2)(DO)binary system.Then,the tracks and M-DNA walker were confined on the Pt HNSs simultaneously to promote the reaction efficiency,whose M-structure boosted the interaction sites between walking strands and tracks and reduced the rigidity of their recognition.Once the CEA approached the sensing interface,the M-DNA walker was activated based on highly specific aptamer recognition to recover ECL intensity with the assistance of exonucleaseⅢ(ExoⅢ).As proof of concept,the“on-off-on”switch aptasensor was constructed for CEA detection with a low detection limit of 0.20 fg/m L.The principle of the constructed ECL aptasensor also enables a universal platform for sensitive detection of other tumor markers.

关 键 词：Platinum hollow nanospheres Electrochemiluminescence aptasensor M-shaped DNA walker On-off-on switch Carcinoembryonic antigen 

分 类 号：Q503[生物学—生物化学] TP212[自动化与计算机技术—检测技术与自动化装置] TB383.1[自动化与计算机技术—控制科学与工程]