林下参内生生防细菌的分离鉴定及定殖能力  

Isolation and identification of endophytic biocontrol bacteria from understory Panax ginseng and study on colonization ability

作　　者：刘子瑶 张岂源 杨平 卢宝慧 杨鹤 许永华[1,2] LIU Ziyao;ZHANG Qiyuan;YANG Ping;LU Baohui;YANG He;XU Yonghua(State Local Joint Engineering Research Center of Ginseng Breeding and Application,Changchun 130118,Jilin,China;College of Traditional Chinese Medicine,Jilin Agricultural University,Changchun 130118,Jilin,China;College of Plant Protection,Jilin Agricultural University,Changchun 130118,Jilin,China)

机构地区：[1]人参新品种选育与开发国家地方联合工程研究中心,吉林长春130118 [2]吉林农业大学中药材学院,吉林长春130118 [3]吉林农业大学植物保护学院,吉林长春130118

出　　处：《微生物学通报》2023年第6期2519-2531,共13页Microbiology China

基　　金：吉林省科技厅重大科技专项(20200504004YY);吉林省科技发展计划(20210401092YY)。

摘　　要：【背景】多年生林下参在自然环境下生长多年,其体内存在的内生菌具有更强的适应性和定殖性,可以提高植物自身抗性,抑制病原菌的生长,更好地发挥与植物的互作。【目的】筛选定殖能力强、繁殖能力快且对病原菌具有拮抗作用的优势菌株。【方法】采用常规组织分离方法,从健康林下参根部组织中分离内生菌,通过对峙试验筛选出对人参病原菌有拮抗作用的内生细菌并对其以传统的鉴定方法进行鉴定。【结果】在得到的6株内生细菌中,菌株LXS-N2对人参立枯病病原菌、人参猝倒病病原菌均有明显抑菌性,而且具有定殖性好、繁殖快的特点,通过破坏病原真菌细胞壁和细胞膜以及改变菌丝形态从而抑制病原真菌生长。【结论】经形态学观察、生理生化反应及16S rRNA基因序列分析鉴定内生菌LXS-N2为贝莱斯芽孢杆菌,具有良好的应用开发潜力。[Background]Understory Panax ginseng grows in the natural environment for many years,and the endogenous bacteria present in its body have stronger adaptability and colonization,which can better play the role of interacting with plants to improve plant resistance and inhibit the growth of pathogenic bacteria.[Objective]To select the dominant strains with strong colonization ability,fast reproductive ability,and antagonistic effects on pathogenic bacteria,understory P.ginseng became the optimal choice.[Methods]The conventional tissue isolation method was used to isolate endophytic bacteria from the root tissues of healthy understory P.ginseng,and the endophytic bacteria with antagonistic effects on ginseng pathogens were screened out by the confrontation test and identified by traditional identification methods.[Results]Among the 6 endophytic bacteria obtained,the strain LXS-N2 had obvious bacteriostatic effects against Rhizoctonia solani and Pythium debaryanum,and it had the characteristics of good colonization and fast reproduction.The strain LXS-N2 inhibited pathogenic fungal growth by destroying the cell wall and membrane of pathogenic fungi and changing hyphae morphology.[Conclusion]The endophytic LXS-N2 was identified as Bacillus velezensis by morphological observation,physiological biochemical reaction,and 16S rRNA gene sequence analysis,which has good application development potential.

关 键 词：林下参 贝莱斯芽孢杆菌 立枯丝核菌 德巴利腐霉菌 分离鉴定 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S435.675[农业科学—植物保护]