Calcium-dependent activation of CPK12 facilitates its cytoplasm-to-nucleus translocation to potentiate plant hypoxia sensing by phosphorylating ERF-Ⅶ transcription factors  被引量:2

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作  者:Biao Fan Ke Liao Lin-Na Wang Li-Li Shi Yi Zhang Ling-Jing Xu Ying Zhou Jian-Feng Li Yue-Qin Chen Qin-Fang Chen Shi Xiao 

机构地区:[1]State Key Laboratory of Biocontrol,Guangdong Provincial Key Laboratory of Plant Resources,chool of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China

出  处:《Molecular Plant》2023年第6期979-998,共20页分子植物(英文版)

基  金:supported by the National Natural Science Foundation of China(Projects 31725004,U22A20458);the Key Realm Research and Development Program of Guangdong Province(Project 2020B0202090001);the Natural Science Foundation of Guangdong Province(Project 2023A1515012038).

摘  要:Calcium-dependent protein kinases(CDPKs/CPKs)are key regulators of plant stress signaling that translate calcium signals into cellular responses by phosphorylating diverse substrate proteins.However,the molecular mechanism by which plant cells relay calcium signals in response to hypoxia remains elusive.Here,we show that one member of the CDPK family in Arabidopsis thaliana,CPK12,is rapidly activated during hypoxia through calcium-dependent phosphorylation of its Ser-186 residue.Phosphorylated CPK12 shuttles from the cytoplasm to the nucleus,where it interacts with and phosphorylates the group Ⅶ ethylene-responsive transcription factors(ERF-Ⅶ)that are core regulators of plant hypoxia sensing,to enhance their stabilities.Consistently,CPK12 knockdown lines show attenuated tolerance of hypoxia,whereas transgenic plants overexpressing CPK12 display improved hypoxia tolerance.Nonethelss,loss of function of five ERF-Ⅶ proteins in an erf-vii pentuple mutant could partially suppress the enhanced hypoxia-tolerance phenotype of CPK12-overexpressing lines.Moreover,we also discovered that phosphatidic acid and 14-3-3κ protein serve as positive and negative modulators of the CPK12 cytoplasm-to-nucleus translocation,respectively.Taken together,these findings uncover a CPK12-ERF-Ⅶ regulatory module that is key to transducing calcium signals from the cytoplasm into the nucleus to potentiate hypoxia sensing in plants.

关 键 词:CPK12 hypoxia sensing cytoplasm-to-nucleus translocation ERF-Ⅶtranscription factors phospha-tidic acid 14-3-3 proteins 

分 类 号:Q94[生物学—植物学]

 

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