Phase separation of the nuclear pore complex facilitates selective nuclear transport to regulate plant defense against pathogen and pest invasion  

作　　者：Jiaojiao Wang Gaofeng Pei Yupei Wang Dewei Wu Xiaokang Liu Gaoming Li Jianfang He Xiaolin Zhang Xiaoyi Shan Pilong Li Daoxin Xie 

机构地区：[1]MOE Key Laboratory of Bioinformatics,Tsinghua-Peking Joint Center for Life Sciences,and Schoolof Life Sciences,Tsinghua University,Beijing 100084,China [2]Bejing Advanced Innovation Center for Structural Biology&Frontier Research Center for Biological Structure,Beijing 100084,China [3]College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009,China [4]Shenzhen Branch of Guangdong Laboratory for Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518124,China

出　　处：《Molecular Plant》2023年第6期1016-1030,共15页分子植物（英文版）

基　　金：supported by funding from the National Natural Science Foundation of China(32250001 and 31830008 to D.X.,32150023 and 32125010 to P.L.,and 31901570 to D.W.);the National Key Research and Development Program of China(2019YFA0508403 to P.L.);the China Postdoctoral Science Foundation(2018M631447 to J.W.);a postdoctoral fellowship of the Tsinghua-Peking Joint Center for Life Sciences.

摘　　要：The nuclear pore complex(NPC),the sole exchange channel between the nucleus and cytoplasm,is composed of several subcomplexes,among which the central barrier determines the permeability/selectivity of the NPC to dominate the nucleocytoplasmic trafficking essential for many important signaling events in yeast and mammals.How plant NPC central barrier controls selective transport is a crucial question remaining to be elucidated.In this study,we uncovered that phase separation of the central barrier is critical for the permeability and selectivity of plant NPC in the regulation of various biotic stresses.Phenotypic assays of nup62 mutants and complementary lines showed that NUP62 positively regulates plant defense against Botrytis cinerea,one of the world’s most disastrous plant pathogens.Furthermore,in vivo imaging and in vitro biochemical evidence revealed that plant NPC central barrier undergoes phase separation to regulate selective nucleocytoplasmic transport of immune regulators,as exemplified by MPK3,essential for plant resistance to B.cinerea.Moreover,genetic analysis demonstrated that NPC phase separation plays an important role in plant defense against fungal and bacterial infection as well as insect attack.These findings reveal that phase separation of the NPC central barrier serves as an important mechanism to mediate nucleocytoplasmic transport of immune regulators and activate plant defense against a broad range of biotic stresses.

关 键 词：nuclear pore complex phase separation plant defense MPK3 

分 类 号：Q94[生物学—植物学]