Transcriptome-wide profiling of RNA N^(4)-cytidine acetylation in Arabidopsis thaliana and Oryza sativa  

作　　者：Bin Li Donghao Li Linjun Cai Qiting Zhou Cong Liu Jianzhong Lin Yixing Li Xiaoying Zhao Li Li Xuanming Liu Chongsheng He 

机构地区：[1]College of Biology,Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation,Hunan Engineering and Technology Research Center of Hybrid Rapeseed,Hunan University,Changsha,410082 Hunan 410082,China [2]State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Hunan Academy of Agricultural Sciences,Changsha,China

出　　处：《Molecular Plant》2023年第6期1082-1098,共17页分子植物（英文版）

基　　金：support from the National Natural Science Foundation of China(32070613,32270623);the Science and Technology Innovation Program of Hunan Province(2021RC3045);support from the National Natural Science Foundation of China(U20A2029);support from the Postgraduate Scientific Research Innovation Project of Hunan Province(CX20200468).

摘　　要：Acetylation of N^(4)-cytidine(ac^(4)C)has recently been discovered as a novel modification of mRNA.RNA ac^(4)C modification has been shown to be a key regulator of RNA stability,RNA translation,and the thermal stress response.However,its existence in eukaryotic mRNAs is still controversial.In plants,the existence,distribution pattern,and potential function of RNA ac^(4)C modification are largely unknown.Here we report the presence of ac^(4)C in the mRNAs of both Arabidopsis thaliana and rice(Oryza sativa).By comparing two ac^(4)C sequencing methods,we found that RNA immunoprecipitation and sequencing(acRIP-seq),but not ac^(4)C sequencing,was suitable for plant RNA ac^(4)C sequencing.We present transcriptome-wide atlases of RNA ac^(4)C modification in A.thaliana and rice mRNAs obtained by acRIP-seq.Analysis of the distribution of RNA ac^(4)C modifications showed that ac^(4)C is enriched near translation start sites in rice mRNAs and near translation start sites and translation end sites in Arabidopsis mRNAs.The RNA ac^(4)C modification level is positively correlated with RNA half-life and the number of splicing variants.Similar to that in mammals,the translation efficiency of ac^(4)C target genes is significantly higher than that of other genes.Our in vitro translation results confirmed that RNA ac^(4)C modification enhances translation efficiency.We also found that RNA ac^(4)C modification is negatively correlated with RNA structure.These results suggest that ac^(4)C is a conserved mRNA modification in plants that contributes to RNA stability,splicing,translation,and secondary structure formation.

关 键 词：N^(4)-acetylcytidine epitranscriptome Arabidopsis thaliana Oryza sativa 

分 类 号：Q94[生物学—植物学]