二穗短柄草BdSKIP基因的克隆及功能分析  

Cloning and functional analysis of BdSKIP gene from Brachypodium distachyon

作　　者：冯金林[1] 席晓宇 李卓宣 柴建祥 FENG Jinlin;XI Xiaoyu;LI Zhuoxuan;CHAI Jianxiang(College of Life Sciences,Shanxi Normal University,Taiyuan 030031,China)

机构地区：[1]山西师范大学生命科学学院,太原030031

出　　处：《植物生理学报》2023年第5期852-860,共9页Plant Physiology Journal

基　　金：山西省基础研究计划自然科学研究面上项目(202203021211261)。

摘　　要：转录和转录后水平的基因表达调控在植物的生长发育和环境响应中发挥重要作用。在酵母和人细胞中,Ski相互作用蛋白(Ski-interacting protein,SKIP)作为转录调节因子和剪接因子分别在转录和转录后水平调节基因表达。本研究克隆了二穗短柄草BdSKIP基因,生物信息学分析表明,BdSKIP蛋白具有典型的SNW结构域,与普通小麦和大麦的SKIP蛋白同源性最高。将BdSKIP基因转化拟南芥skip-1突变体,结果显示BdSKIP蛋白在维持主根正常生长和抑制拟南芥提前开花方面发挥重要的作用,并且可以在转录和转录后水平调控基因的表达。亚细胞定位结果显示,BdSKIP蛋白定位于细胞核,与调控基因表达的功能相一致。本研究揭示了BdSKIP蛋白的功能,为BdSKIP蛋白的进一步应用提供了基础。Transcriptional and post-transcriptional gene expression regulation plays an important role in plant growth and development and environmental response.In yeast and human cells,Ski-interacting protein(SKIP)acts as a transcription regulator and a splicing factor to regulate gene expression at the transcriptional and post-transcriptional levels,respectively.In this study,BdSKIP gene of Brachypodium distachyon was cloned.Bioinformatics analysis showed that the BdSKIP protein has a typical SNW domain and has the highest homology with the SKIP proteins in Triticum aestivum and Hordeum vulgare.BdSKIP gene was transformed into Arabidopsis skip-1 mutant.The results showed that BdSKIP protein played an important role in maintaining the normal growth of primary roots and inhibiting the early flowering of Arabidopsis,and can regulate gene expression at the transcriptional and post-transcriptional levels.The subcellular localization results showed that BdSKIP protein was located in the nucleus,which was consistent with the function of regulating gene expression.This study revealed the function of BdSKIP protein and provided a basis for further application of the protein.

关 键 词：二穗短柄草 SKIP 剪接因子 转录调节因子 

分 类 号：Q943.2[生物学—植物学]