葡萄GH5基因家族全基因组鉴定及生物信息学分析  

Whole genome identification and bioinformatics analysis of grape GH5 gene family

作　　者：黎霖 聂鸿飞 叶文秀 赵旗峰 纪薇[1,2,3] LI Lin;NIE Hongfei;YE Wenxiu;ZHAO Qifeng;JI Wei(College of Horticulture,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China;Yongji County Agricultural and Rural Bureau,Yuncheng,Shanxi 044500,China;Institute of Modern Agriculture,Peking University,Weifang Modern Agriculture Shandong Province Laboratory,Weifang Key Laboratory of Grapes Germplasm Innovation and Utilization,Weifang,Shandong 261000,China;Institute of Fruit Tree Research,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China)

机构地区：[1]山西农业大学园艺学院,山西晋中030801 [2]运城市县农业农村局,山西运城044500 [3]北京大学现代农业研究院,潍坊市葡萄种质创新与利用重点实验室,潍坊现代农业山东省实验室,山东潍坊261000 [4]山西农业大学果树研究所,山西晋中030801

出　　处：《植物生理学报》2023年第5期909-922,共14页Plant Physiology Journal

基　　金：山西省科技成果转化引导专项项目(202204021301028);山西省高等学校优秀成果(科学技术)培育项目(2020KJ037);山西省研究生教育创新计划项目(2022Y335);山西省果树产业技术体系(2023CYJSTX07-01)。

摘　　要：纤维素酶是一种能将纤维素分解成寡糖或单糖的蛋白质。在糖苷水解酶家族中,GH5基因家族是植物当中功能最多样化且数量最多的植物纤维素酶。本研究鉴定到分布在葡萄5条染色体上的18个GH5家族基因。进化树分析显示葡萄的GH5家族基因分为3个亚家族,同在一个进化分类的成员其基因结构相似。通过基因结构分析发现葡萄GH5家族成员含1~6个内含子,内含子与外显子数量差异较小。亚细胞定位分析发现葡萄GH5家族成员主要分布在细胞核。蛋白结构分析发现葡萄GH5家族成员以α-螺旋和无规则卷曲为主。顺式作用元件分析发现葡萄VvGH5基因上游启动子序列包含大量的激素响应元件,暗示这些基因家族成员在响应植物内源激素调控中有重要作用。本研究将为深入研究葡萄GH5家族成员的基因功能和分子育种工作提供理论基础。Cellulase is a kind of protein that can decompose cellulose into oligosaccharides or monosaccharides.Among the glycoside hydrolase families,GH5 gene family is the plant cellulase with the most diverse functions and the largest number in plants.In this study,18 GH5 family genes distributed on 5 chromosomes of grape were identified.Phylogenetic tree analysis showed that the GH5 family genes of grapes were divided into three subfamilies,which were similar to the members of the same evolutionary classification.Through gene structure analysis,it was found that grape GH5 family members contain 1−6 introns,and there is little difference in the number of introns and exons.Subcellular localization analysis showed that grape GH5 family members were mainly distributed in the nucleus.Protein structure analysis showed that grape GH5 family membersα-spiral and irregular curl are dominant.Through homeopathic element analysis,it was found that the upstream promoter sequence of grape VvGH5 gene contained a large number of hormone response elements,it is suggested that these gene family members play an important role in response to plant endogenous hormone regulation.This study will provide a theoretical basis for indepth study of gene function and molecular breeding of grape GH5 family members.

关 键 词：葡萄 GH5基因家族 生物信息学 

分 类 号：S663.1[农业科学—果树学]