铜绿假单胞菌动物感染模型的构建及研究进展  

Constructions and advances of animal models of Pseudomonas aeruginosa infection

作　　者：任彦颖 刘莹[1] 费冰 郭梦雨 刘心伟 刘冬梅 李永伟[1] Ren Yanying;Liu Ying;Fei Bing;Guo Mengyu;Liu Xinwei;Liu Dongmei;Li Yongwei(The Second Clinical Medical College of Henan University of Chinese Medicine,Zhengzhou 450001,China;Medicine Laboratory Center of Henan Province Hospital of Traditional Chinese Medicine,Henan Provincial Key Laboratory of Antibiotics-Resistant Bacterial Infection Prevention&Therapy with Traditional Chinese Medicine,The Key Laboratory of Pathogenic Microbes&Antimicrobial Resistance Surveillance of Zhengzhou,Zhengzhou 450002,China)

机构地区：[1]河南中医药大学第二临床医学院,郑州450001 [2]河南省中医院检验中心,河南省防治耐药菌感染中医药重点实验室,郑州市病原微生物与细菌耐药监测重点实验室,郑州450002

出　　处：《中华预防医学杂志》2023年第6期929-934,共6页Chinese Journal of Preventive Medicine

基　　金：河南省自然科学基金面上项目(202300410263);河南省中医药科学研究专项(2022ZY1078);河南省高等学校重点科研项目(22A360008)。

摘　　要：铜绿假单胞菌是一种致病机制和耐药机制复杂、发病率和死亡率高的机会致病菌,可致免疫功能低下个体各种组织部位的急、慢性感染,临床以肺部感染、伤口感染和血流感染最为常见。铜绿假单胞菌动物感染模型通过模拟人体细菌感染途径,对深入研究铜绿假单胞菌致病性、耐药性及其防治措施具有重要的应用价值。本文就铜绿假单胞菌动物感染模型构建中实验动物的选择、麻醉和处置进行概述,并对铜绿假单胞菌肺部感染、伤口感染和血流感染动物模型的构建方法、模型评估以及应用进行阐述,以期为铜绿假单胞菌感染性疾病相关科学研究工作提供参考。Pseudomonas aeruginosa(P.aeruginosa)is an opportunistic pathogenic bacterium with complex pathogenesis and drug resistance mechanisms.It has high morbidity and mortality and can cause acute and chronic infections in immunocompromised individuals,with lung infections,wound infections,and bloodstream infections being the most common.The animal infection model of P.aeruginosa is of great value for in-depth research on the pathogenicity,drug resistance,and therapeutic measures of P.aeruginosa by simulating the pathways of human bacterial infections.This article firstly summarizes the selection,anesthesia,and disposal of experimental animals in the construction of animal models of P.aeruginosa infection,and then reviews the methods of construction,model evaluation,and applications of animal models of P.aeruginosa pulmonary infection,wound infection,and bloodstream infection,in order to provide a reference for scientific research related to P.aeruginosa infectious diseases.

关 键 词：铜绿假单胞菌 模型 动物 细菌感染 

分 类 号：R446.5[医药卫生—诊断学] R-332[医药卫生—临床医学]