Characterisation and separation of infectious bursal disease virus-like particles using aqueous two-phase systems  

作　　者：Hui Yi Leong Xiao-Qian Fu Xiang-Yu Liu Shan-Jing Yao Dong-Qiang Lin 

机构地区：[1]Zhejiang Key Laboratory of Smart Biomaterials,Key Laboratory of Biomass Chemical Engineering of Ministry of Education,College of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310027,China [2]Sinopharm Animal Health Corporation Ltd.,Wuhan 430075,China

出　　处：《Chinese Journal of Chemical Engineering》2023年第5期72-78,共7页中国化学工程学报（英文版）

基　　金：Zhejiang University and TalentIntroduction Program of China for Postdoctoral Researcher for the financial support;financially supported by the National Key Research&Development Program of China (2021YFE0113300);the National Natural Science Foundation of China (22078286)。

摘　　要：Infectious bursal disease(IBD)causes considerable economic losses in the commercial poultry industry worldwide.The principal way to control IBD virus(IBDV),the causative agent of IBD,is still through vaccination programs.Virus-like particles(VLPs)are recognised as a safe and potent recombinant vaccine platform.This research work explores the characterisation and separation of infectious bursal disease virus-like particles(IBD-VLPs)from crude feedstock.Various characteristics were studied with highperformance size-exclusion chromatography(HP-SEC),sodium dodecyl sulphate–polyacrylamide gel electrophoresis(SDS-PAGE)and transmission electron microscopy(TEM)analyses.Subsequently,the separation of IBD-VLPs using polyethylene glycol(PEG)/sodium citrate-based aqueous two-phase systems(ATPSs)was conducted and optimised.Moreover,a scale-up study of the best ATPS constituted of 15%PEG 6000,11%sodium citrate and 10%crude feedstock was performed to compare the separation performance of IBD-VLPs with and without centrifugation-assisted.The results indicated that the optimised ATPS with centrifugation-assisted for both 5 g and 50 g systems showed good recovery of IBDVLPs of>97%in the interphase between the PEG-rich top and salt-rich bottom phases.These optimised systems also showed high removal efficiencies of impurities of>95%.The results demonstrated that aqueous two-phase extraction could be a promising technology for efficient VLPs separation.

关 键 词：Aqueous two-phase extraction Infectious bursal disease virus POLYMERS SALT SEPARATION Virus-like particle 

分 类 号：S859.797[农业科学—临床兽医学] TQ028[农业科学—兽医学]