参麦注射液联合盐酸安罗替尼对A549细胞的干预机制  

Intervention mechanism of Shenmai Injection combined with Anrotinib Hydrochloride on A549 Cells

作　　者：王晓芳 杨雪洁 舒健峰 WANG Xiaofang;YANG Xuejie;SHU Jianfeng(Ningbo Institute of Life and Health Industy,University of Chinese Academy of Sciences,Zhejiang Province,Ningbo315000,China)

机构地区：[1]国科宁波生命与健康产业研究院,浙江宁波315000

出　　处：《中国当代医药》2023年第19期12-16,共5页China Modern Medicine

基　　金：浙江省宁波市医学科技计划项目(2020Y24)。

摘　　要：目的探讨参麦注射液(SMI)联合盐酸安罗替尼(Anl)对人肺癌A549细胞的干预机制。方法不同浓度的SMI干预A549细胞24 h,通过CCK-8法检测细胞的存活率;不同浓度的Anl及不同浓度的Anl联合SMI IC_(50)分别干预A549细胞24 h,通过CCK-8法检测细胞的存活率。将A549细胞分为四组:空白组,低浓度组(SMI 9 mg/ml+Anl IC_(50)),中浓度组(SMI 18 mg/ml+Anl IC_(50))和高浓度组(SMI 36 mg/ml+Anl IC_(50));采用JC-1荧光染色实验检测各组细胞线粒体膜电位的变化,采用Western blot检测撕裂蛋白1(Mnf1)及动力相关蛋白(Drp1)的表达。结果CCK-8法显示SMI IC_(50)的浓度为36.10 mg/ml,各SMI用药组与空白组比较,差异有统计学意义(P<0.01);Anl单独用药与空白组比较,差异有统计学意义(P<0.01);SMI联合Anl用药与空白组比较,差异有统计学意义(P<0.01)。在SMI的协同作用下Anl的IC_(50)值由单独用药的1.006μmol/L降至0.84μmol/L。JC-1荧光染色结果显示,与空白组比较,低浓度组、中浓度组、高浓度组线粒体膜电位下降,差异有统计学意义(P<0.01);Western blot检测Mfn1表达结果显示,低浓度组与空白组比较,差异有统计学意义(P<0.05);中浓度组和高浓度组与空白组比较,差异有统计学意义(P<0.01)。Drp1蛋白表达结果显示,低浓度组与空白组比较,差异无统计学意义(P>0.05);中浓度组与空白组比较,差异有统计学意义(P<0.05);高浓度组与空白组比较,差异有统计学意义(P<0.01)。结论SMI联合Anl协同抑制A549细胞存活,并通过线粒体途径促进A549细胞凋亡。Objective To explore the intervention mechanism of Shenmai Injection(SMI)combined with Anlotinib Hydrochloride(Anl)on human lung cancer A549 cells.Methods Different concentrations of SMI were used to intervene in A549 cells for 24 hours,the cell survival rate was measured using the CCK-8 method.Different concentrations of Anl and different concentrations of Anl combined with SMI IC_(50) intervened with A549 cells for 24 hours,the cell survival rate was measured using the CCK-8 method.A549 cells were divided into four groups:blank group,low concentration group(SMI 9 mg/ml+Anl IC_(50)),medium concentration group(SMI 18 mg/ml+Anl IC_(50)),and high concentration group(SMI 36 mg/ml+Anl IC_(50)).JC-1 fluorescent staining test was used to detect the changes of mitochondrial membrane potential in each group,and Western blot was used to detect the expression of mitofusin 1(Mnf1)and dynamin-related protein 1(Drp1).Results The CCK-8 method showed that the concentration of SMI IC_(50) was 36.10 mg/ml,and the difference between the SMI treatment groups and the blank group was statistically significant(P<0.01).There was statistically significant difference between the Anl alone group and the blank group(P<0.01).There was statistically significant difference between SMI combined with Anl group and the blank group(P<0.01).Under the synergistic effect of SMI,the IC_(50) value of Anl droppd from 1.006μmol/L when administered alone to 0.84μmol/L.JC-1 fluorescence staining results showed that compared with the blank group,the mitochondrial membrane potential in the low concentration group,the medium concentration group and the high concentration group decreased,the differences were statistically significant(P<0.01).The expression of Mfn1 was detected by Western blot,and the difference between the low concentration group and the blank group was statistically significant(P<0.05).The differences between the medium concentration group and the high concentration group and the blank group were statistically significant(P<0.01).The expre

关 键 词：肺癌 参麦注射液 盐酸安罗替尼 线粒体融合蛋白1 动力相关蛋白1 

分 类 号：R285[医药卫生—中药学]