激光辅助孵化对透明带增厚胚胎囊胚发育潜能的影响  

Effect of laser-assisted hatching on the blastocyst developmental potential of embryos with thickened zona pellucida

作　　者：刘树沅[1] 罗鑫 蔡昌明 林秀峰[1] LIU Shuyuan;LUO Xin;CAI Changming;LIN Xiufeng(Reproductive Medical Center,Boai Hospital of Zhongshan,Guangdong Province,Zhongshan528403,China)

机构地区：[1]广东省中山市博爱医院生殖中心,广东中山528403

出　　处：《中国当代医药》2023年第19期81-85,共5页China Modern Medicine

基　　金：广东省中山市社会公益科技研究项目(2019B1025)。

摘　　要：目的探讨新鲜周期中激光辅助孵化(LAH)对透明带增厚胚胎囊胚发育潜能的影响。方法选取2019年8月至2021年12月在中山市博爱医院生殖中心行辅助生殖技术(ART)治疗且胚胎透明带增厚的患者为研究对象。根据患者意愿将患者分为LAH组(85例,胚胎数776个)与对照组(92例,胚胎数772个),每组进一步分为非高龄组(<35岁)和高龄组(≥35岁)。LAH组患者采用LAH技术,全部可用胚胎进行透明带削薄处理后再进行培养;对照组胚胎透明带不进行辅助孵化处理直接进行培养。比较各年龄段患者中LAH组和对照组的囊胚形成情况及临床结局。结果非高龄患者中,LAH组的优质囊胚率和囊胚孵出率均高于对照组,差异有统计学意义(P<0.05);高龄患者中,LAH组和对照组的优质囊胚率及囊胚孵出率比较,差异无统计学意义(P>0.05);LAH组与对照组在不同年龄段的临床妊娠率、着床率、流产率和活产率比较,差异无统计学意义(P>0.05)。结论在新鲜周期中,非高龄患者透明带增厚的胚胎行LAH后有利于优质囊胚的形成与囊胚孵出。Objective To explore the effect of laser-assisted hatching(LAH)on the blastocyst developmental potential of embryos with thickened zona pellucida in fresh cycles.Methods Patients who received assisted reproductive technology(ART)treatment and embryo zona pellucida thickened in the Reproductive Medical Center,Boai Hospital of Zhongshan from August 2019 to December 2021 were selected as the study objects.Patients were divided into LAH group(85 cases,776 embryos)and control group(92 cases,772 embryos)according to their wishes,and each group was further divided into non-elderly group(<35 years old)and elderly group(≥35 years old).LAH group was treated with LAH technology,and all the embryos were used for zona pellucida thinning before culture.In the control group,the embryo zona pellucida was cultured directly without assisted incubation.Blastocyst formation and clinical outcome were compared between LAH group and control group.Results Among non-elderly patients,the rates of fine blastocyst and blastocyst incubation in LAH group were higher than those in control group,and the differences were statistically significant(P<0.05).There were no significant difference between LAH group and control group in the rate of fine blastocyst and the rate of blastocyst incubation in elderly patients(P>0.05).There were no significant differences in clinical pregnancy rate,implantation rate,abortion rate and live birth rate between LAH group and control group at different ages(P>0.05).Conclusion In the fresh cycle,the embryo LAH with the thickened zona pellucida in non-elderly patients is conducive to the formation of high-quality blastocyst and blastocyst incubation.

关 键 词：透明带增厚 激光辅助孵化 囊胚培养 囊胚发育潜能 辅助生殖技术 

分 类 号：R711[医药卫生—妇产科学]