芦笋腋芽一步成苗组培技术研究  

Study on One-Step Tissue Culture to Seedlings of Asparagus officinalis L.with Axillary Buds as Explants

作　　者：卢金东[1] 王波[2] 厉广辉 周长生 尹俊玉 王兴军[1] 赵术珍[1] Lu Jindong;Wang Bo;Li Guanghui;Zhou Changsheng;Yin Junyu;Wang Xingjun;Zhao Shuzhen(Institute of Crop Germplasm Resources,Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Crop Genetic Improvement,Ecology and Physiology,Jinan 250100,China;Weifang Engineering Vocational College,Qingzhou 262500,China;Shandong Juxinyuan Agriculture Technology Co.,Ltd.,Caoxian 274400,China;Beijing Academy of Agriculture and Forestry Sciences,Beijing 100081,China)

机构地区：[1]山东省农业科学院农作物种质资源研究所/山东省作物遗传改良与生态生理重点实验室,山东济南250100 [2]潍坊工程职业学院,山东青州262500 [3]山东巨鑫源农业科技有限公司,山东曹县274400 [4]北京市农林科学院,北京100081

出　　处：《山东农业科学》2023年第6期42-46,共5页Shandong Agricultural Sciences

基　　金：山东省农业科技资金(园区产业提升工程)项目“黄河三角洲盐碱地芦笋栽培关键技术集成与应用”(2019YQ004)。

摘　　要：本研究通过对外植体及其消毒方法、生根培养激素组合的筛选,建立了芦笋腋芽一步成苗的组培快繁方法。结果表明,以5~10 mm腋芽为外植体,接种在1/2MS+0.05 mg/L NAA+0.1 mg/L KT+1.0 mg/L嘧啶醇+1.0 mg/L IBA+2%蔗糖+0.65%琼脂的培养基上,30天左右即可生根,45天左右根长可达1 cm;再生根可分为3种类型,即从芽基部长出的粗壮肉质根(Ⅰ型)、从愈伤组织上再生的不定根(Ⅱ型)以及从芽基部长出的细长根(Ⅲ型),在确定的最适生根培养基上产生的根主要是Ⅰ型根。培养60天后芽伸长明显,长度可达3~5 cm;培养70天后,将生长健壮的生根苗进行炼苗后移栽,移栽成活率达98%以上。该方法能够为芦笋优异单株的快速繁殖提供解决方案,为规模化生产F1杂种提供技术支撑。Through screening explants,disinfection methods and hormone combinations for rooting cul-ture,the one-step tissue culture and rapid propagation method for Asparagus officinalis L.with axillary buds as explants was established.The results showed that the 5~10-mm axillary bud explants were inoculated on the medium consist of 1/2MS+0.05 mg/L NAA+0.1 mg/L KT+1.0 mg/L pyrimidine+1.0 mg/L IBA+2%sucrose+0.65%agar,and could root after about 30 days.After cultured for about 45 days,the root length could reach 1 cm.The regenerated roots could be divided into three types including stout fleshy roots growing from the base of axillary buds(typeⅠ),adventitious roots generated from callus(typeⅡ),and slender roots growing from the base of axillary buds(typeⅢ).The roots produced on the optimum rooting medium were mainly typeⅠroots.After 60 days of culture,the shoots elongation was obvious,and the length could reach 3~5 cm.After 70 days of culture,the strong seedlings were acclimated and then transplanted,and the survival rate was over 98%.This method could provide a solution for the rapid propagation of excellent individual plants of A.offici-nalis,and provide technical support for large-scale production of F1 hybrids.

关 键 词：芦笋 腋芽 组织培养 生根 再生 

分 类 号：S644.6[农业科学—蔬菜学]