基于VIGS技术干扰病毒RNA防控烟草黄瓜花叶病毒病  

Control of Tobacco Cucumber Mosaic Virus Disease by Interfering Viral RNA Based on VIGS Technology

作　　者：郭玉鸽 张倩 杨惠娟[1] 李俊营 常栋[2] 张富生 武兆云[1] 阎海涛 杨铁钊[1] Guo Yuge;Zhang Qian;Yang Huijuan;Li Junying;Chang Dong;Zhang Fusheng;Wu Zhaoyun;Yan Haitao;Yang Tiezhao(College of Tobacco,Henan Agricultural University,Zhengzhou 450002,China;Pingdingshan Branch of Henan Tobacco Company,Pingdingshan 467000,China)

机构地区：[1]河南农业大学烟草学院,河南郑州450002 [2]河南省烟草公司平顶山市公司,河南平顶山467000

出　　处：《山东农业科学》2023年第6期136-142,共7页Shandong Agricultural Sciences

基　　金：河南省烟草公司平顶山市公司科技项目(PYKJ202207)。

摘　　要：黄瓜花叶病毒(Cucumber mosaic virus,CMV)引起的花叶病是烟草上的重要病害。本试验通过病毒诱导基因沉默技术(virus induced gene silencing,VIGS)构建靶向沉默CMV-1a、CMV-2a、CMV-MP和CMV-CP的VIGS载体,测定基因沉默后CMV相对表达量、CMV病毒浓度和TRV相对表达量,并对VIGS载体对CMV的防治效果进行评价。结果表明,试验建立的VIGS载体能够有效导入烟株;沉默CMV-2a的处理CMV相对表达量最低,基因沉默效率最高,为46.25%;接种病毒30 d后CMV-2a处理的病毒浓度最低,仅为对照的72.8%,TRV载体的相对表达量显著高于其他处理;沉默CMV-2a的处理发病时间推迟,病情指数最低,防治效果最好。本研究建立的CMV-2a VIGS体系能够有效沉默外源侵入的CMV基因表达,抑制CMV在烟株内的传播,为防治烟草黄瓜花叶病毒病提供了新的思路与方法。Mosaic disease caused by cucumber mosaic virus(CMV)is an important disease of tobacco.This experiment used the virus induced gene silencing(VIGS)technology to construct VIGS vectors targeted silencing CMV-1a,CMV-2a,CMV-MP and CMV-CP.The relative expression level of CMV,the concentration of CMV virus and the relative expression level of TRV after gene silencing were measured,and the preventive and control effects of VIGS vectors against CMV were evaluated.The results showed that the VIGS vectors es-tablished in the experiment could be introduced into tobacco plants effectively.The treatment of silencing CMV-2a had the lowest relative expression level of CMV and the highest gene silencing efficiency as 46.25%.After 30 days of virus inoculation,the CMV-2a treatment had the lowest virus concentration,only 72.8%of that of control,and the relative expression level of TRV vector was significantly higher than that of the other treatments.Silencing CMV-2a treatment delayed the onset time and the disease index was the lowest,so it had the best preventive and control effect.The CMV-2a VIGS system established in this study could effectively si-lence the expression of exogenous CMV genes,and inhibit the transmission of CMV in tobacco plants,which provided a new method for the prevention and control of tobacco cucumber mosaic virus disease.

关 键 词：VIGS 技术 干扰病毒 RNA 烟草 黄瓜花叶病毒病 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治] Q789[农业科学—植物保护]