霍山石斛多糖对Lewis肺癌荷瘤小鼠肿瘤生长的抑制作用  

The Inhibitory Effects of Dendrobium huoshanense Polysaccharides on Tumor Growth in Lewis Lung Carcinoma-bearing Mice

作　　者：张金萍 葛基春 李强明 王成 查学强 罗建平 ZHANG Jinping;GE Jichun;LI Qiangming;WANG Cheng;ZHA Xueqiang;LUO Jianping(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,China;Anhui Huoshan Tianxiazeyu Biotechnology Development Co.Ltd.,Lu'an 237200,China)

机构地区：[1]合肥工业大学食品与生物工程学院,安徽合肥230601 [2]安徽省霍山县天下泽雨生物科技发展有限公司,安徽六安237200

出　　处：《现代食品科技》2023年第7期7-15,共9页Modern Food Science and Technology

基　　金：安徽省科技重大专项(15czz04087)。

摘　　要：该研究探讨了霍山石斛多糖(cDHP)对Lewis肺癌荷瘤小鼠肿瘤生长的抑制作用。用Lewis肺癌细胞建立肺癌荷瘤小鼠模型,观测cDHP对小鼠生长情况、肿瘤体积、抑瘤率和脾淋巴细胞增殖能力的影响,并测定肿瘤细胞凋亡比例、脾脏免疫细胞(T细胞、Treg细胞、B细胞、树突状细胞和巨噬细胞)比例及血清细胞因子(CEA、IL-1β、IL-10、IL-2、TNF-α、VEGF和TGF-β)含量的变化。cDHP高369.00 mg/(kg·d)、中184.50 mg/(kg·d)、低92.25 mg/(kg·d)剂量组的抑瘤率分别为64.52%、50.47%、37.68%。cDHP的抗肿瘤作用表现在其能够提高荷瘤小鼠肿瘤细胞凋亡比例、改善荷瘤小鼠脾淋巴细胞增殖能力、调节荷瘤小鼠脾脏免疫细胞比例和血清细胞因子的平衡。cDHP可通过不同方式影响肿瘤生长,结果为霍山石斛的开发利用提供了实验依据。To investigate the inhibitory effects of cultivated Dendrobium huoshanense polysaccharides(cDHP)on tumor growth in Lewis lung carcinoma(LLC)-bearing mice,a mouse model of lung cancer was established using LLC cells.The effects of cDHP on various parameters including mouse growth,tumor mass,tumor growth inhibition ratio(treatment/control,T/C),and the proliferative capacity of spleen lymphocytes were assessed.Additionally,changes in the percentage of tumor cell apoptosis,composition of spleen immune cells(T cells,Treg cells,B cells,dendritic cells,and macrophages),and levels of serum cytokines(CEA,IL-1β,IL-10,IL-2,TNF-α,VEGF,and TGF-β)were monitored.The high-dose(369.00 mg/(kg·d)),medium-dose(184 mg/(kg·d)),and low-dose(92.25 mg/(kg·d))cDHP groups exhibited T/C ratios of 64.52%,50.47%,and 37.68%,respectively.In lung tumor-bearing mice,cDHP demonstrated antitumor effects through an increase in the percentage of tumor cell apoptosis,enhanced proliferative capacity of spleen lymphocytes,regulation of spleen immune cell composition,and modulation of serum cytokine levels.These findings indicate that cDHP influences tumor growth through multiple mechanisms.The findings of this study provide experimental evidence supporting the potential development of antitumor drugs based on cDHP.

关 键 词：霍山石斛 多糖 抗肿瘤作用 荷瘤小鼠 

分 类 号：R285.5[医药卫生—中药学]