2021—2022年山东省猪繁殖与呼吸综合征病毒分子流行病学调查  

Molecular epidemiological investigation of porcine reproductive and respiratory syndrome virus infection in Shandong Province from 2021 to 2022

作　　者：姜丹丹 王凯月 杜以军[2] 姜平 JIANG Dandan;WANG Kaiyue;DU Yijun;JIANG Ping(Key Laboratory of Bacteriology,Ministry of Agriculture and Rural Affairs/College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences/Shandong Province Key Laboratory of Animal Disease Control and Breeding,Jinan 250100,China;College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300380,China)

机构地区：[1]南京农业大学动物医学院/农业农村部动物细菌学重点实验室,江苏南京210095 [2]山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室,山东济南250100 [3]天津农学院动物科学与动物医学学院,天津300380

出　　处：《畜牧与兽医》2023年第6期87-92,共6页Animal Husbandry & Veterinary Medicine

基　　金：山东省自然科学基金(ZR2020QC196,ZR2020KC005,ZR2021MC139,ZR2021ZD08);国家自然科学基金(32102710)。

摘　　要：为研究2021—2022年猪繁殖与呼吸综合征病毒(PRRSV)在山东地区的流行情况,从疑似发病的猪场采集了103份临床样本,采用RT-PCR方法对样品进行检测,并对阳性样品进行GP5和NSP2基因的序列测定、遗传进化分析以及同源性分析。结果显示:103份样品中检测到21份阳性样品,阳性率为20.38%。最终有5个样品能够同时扩增出GP5和NSP2基因序列,遗传进化分析显示,这5个样品中有2个属于谱系(lineage)1;2个属于lineage 5;1个属于lineage 8。各样品之间GP5基因的核苷酸同源性为81.5%~99.3%,NSP2基因的核苷酸同源性为56.9%~99.4%。本研究表明2021—2022年山东地区的PRRSV传播呈现多样性,为PRRSV毒株的遗传变异、演化分析和防控提供了理论基础。To study the prevalence of porcine reproductive and respiratory syndrome virus(PRRSV)in Shandong Province from 2021 to 2022,103 clinical samples were collected of the suspected disease afflicting pigs in different farms.RT-PCR was used to detect the presence of PRRSV in the samples.The results showed that 21 samples were positive,at a positive rate of 20.38%.Then,the GP5 and NSP2 genes of 5 PRRSV strains were amplified and sequenced at the same time.The genetic evolution analysis showed that two strains belonged to lineage 1,another two strains belonged to lineage 5 and one strain belonged to lineage 8.The nucleotide homology of the GP5 and NSP2 genes of the 5 PRRSV strains were 81.5%-99.3% and 56.9%-99.4%,respectively.Our study showed that PRRSV transmission in Shandong Province from 2021 to 2022 was of diversity,which provided a theoretical basis for the genetic variation,evolutionary analysis,and prevention and control of PRRSV.

关 键 词：猪繁殖与呼吸综合征病毒 同源性 流行病学 遗传变异 进化分析 

分 类 号：S852.6[农业科学—基础兽医学]