高效毛细管电泳法检测淡水鱼肉中阿莫西林和氨苄西林残留方法的建立  

Establishment of a High Performance Capillary Electrophoresis Method for the Determination of Amoxicillin and Ampicillin Residues in Freshwater Fish

作　　者：孙雪峰 王方雨 SUN Xuefeng;WANG Fangyu(Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450000,China)

机构地区：[1]河南省农业科学院动物免疫学重点实验室,河南郑州450000

出　　处：《肉类研究》2023年第6期29-33,共5页Meat Research

基　　金：“十三五”国家重点研发计划重点专项(2019YFC1605701)。

摘　　要：为建立一种基于高效毛细管电泳技术、可同时高效检测淡水鱼肉中阿莫西林和氨苄西林残留的高通量检测方法,选用鲢鱼作为淡水鱼的代表,以空白鱼肉为试材,使用二极管阵列检测器进行检测。结果表明:样品经乙腈提取、旋转蒸发仪浓缩、固相萃取柱萃取后,在pH 7.85的30 mmol/L硼砂溶液缓冲体系中,检测波长210 nm、检测电压20 kV、检测温度25℃的电泳条件下,阿莫西林和氨苄西林在20 min内能够实现基线分离;在0.02~10.00μg/mL质量浓度范围内,阿莫西林和氨苄西林的峰面积与质量浓度之间呈线性相关,相关系数(R^(2))均大于0.990;阿莫西林在淡水鱼肉中的加标回收率为78.90%~85.72%,日内变异系数小于8.01%,氨苄西林在淡水鱼肉中的加标回收率为75.36%~86.34%,日内变异系数小于8.06%。This study established a high-throughput method for the simultaneous and efficient detection of amoxicillin and ampicillin residues in silver carp as a representative of freshwater fish using high performance capillary electrophoresis(HPCE)with a diode array detector.Samples were extracted with acetonitrile,concentrated by rotary evaporation and cleaned up using a solid phase extraction(SPE)column.The capillary column was washed with 30 mmol/L borax buffer solution at pH 7.85.The detection wavelength,voltage and temperature were set at 210 nm,20 kV and 25℃,respectively.Baseline separation of amoxicillin and ampicillin was accomplished within 20 min.The peak area of amoxicillin and ampicillin showed a linear correlation with their concentrations in the range of 0.02-10.00μg/mL,with correlation coefficients(R^(2))greater than 0.990.The recoveries of amoxicillin and ampicillin spiked in freshwater fish were 78.90%-85.72% and 75.36%-86.34%,respectively,with within-day coefficient of variation(CV)less than 8.01%and 8.06%,respectively.

关 键 词：高效毛细管电泳 阿莫西林 氨苄西林 残留 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]