生物组织及其细胞溶液介电谱测量研究  

The dielectric spectrum measurement of tissues and cell solutions

作　　者：黄伟伟 胡亚楠 岑泽南 朱金俊 朱朝阳 包家立 HUANG Weiwei;HU Yanan;CEN Zenan;ZHU Jinjun;ZHU Chaoyang;BAO Jiali(Research Team of Biophysics and Medical Engineering,Zhejiang Provincial Key Laboratory of Bioelectromagnetics,School of Medicine,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学医学院,浙江省生物电磁学重点实验室生物物理与医学工程研究组,杭州310058

出　　处：《生物医学工程研究》2023年第2期152-158,共7页Journal Of Biomedical Engineering Research

基　　金：国家科技重大专项资助项目(2018ZX10301201-002)。

摘　　要：为阐明生物组织中细胞及组织液等电介质介电谱之间的相互关系,本研究测量了肝组织、肝细胞悬液(BRL)、肝癌细胞悬液(RH-35)、细胞培养基(dulbecco′s modified eagle medium,DMEM)、磷酸缓冲盐溶液(phosphate buffered saline,PBS)、超纯水的介电谱。测量结果显示,肝组织和超纯水介电损耗谱ε″最大值分别为6.18×10^(6)和2.60×10^(4),测量精度为9.03%和9.52%,相对介电常数谱ε′最大值分别为1.96×10^(6)和1.80×10^(3),测量精度为16.85%和12.97%;BRL、RH-35、DMEM、PBS的ε″具有峰现象,最大值分别为5.00×10^(5)、4.40×10^(5)、3.70×10^(5)、5.90×10^(5),测量精度RH-35为5.44%,其他不超过2%;ε′最大值分别为1.90×10^(6)、1.80×10^(6)、1.40×10^(6)、2.00×10^(6),测量精度RH-35为2.34%,其他不超过2.00%。结果表明,生物组织介电损耗谱ε″和相对介电常数谱ε′随频率呈单调下降,测量精度较低;而生物溶液介电损耗谱ε″呈峰现象,具有弛豫性,相对介电常数谱ε′随频率呈单调下降,测量精度较高;生物组织中的有极细胞可消耗较多的电场能量,是产生电磁生物效应的重要因素。In order to clarify the relationship between the dielectric spectra of cells and tissue fluid in biological tissues,dielectric spectra of liver tissue,BRL,RH-35,dulbecco′s modified eagle medium(DMEM),phosphate buffered saline(PBS)and water were measured.The measurement results showed that the maximum dielectric lossε″of liver tissue and ultrapure water were 6.18×106 and 2.60×10^(4),with measurement accuracy of 9.03%and 9.52%,respectively.The maximum values of the relative dielectric constantε′were 1.96×10^(6)and 1.80×10^(3),with measurement accuracy of 16.85%and 12.97%,respectively.ε″of BRL,RH-35,DMEM and PBS had a peak,the maximum values ofε″were 5.00×10^(5),4.40×10^(5),3.70×10^(5)and 5.90×10^(5),respectively,the maximum value ofε′were 1.90×10^(6),1.80×10^(6),1.40×10^(6)and 2.00×10^(6),respectively.The measurement accuracy ofε″andε′of RH-35 were 5.44%and 2.34%,the others were less than 2.00%.The results showed thatε″andε′of biological tissue decreases monotonously in frequency dependence with low accuracy.Whileε″of biological solution showes peak phenomenon and relaxation properties,ε′decreases monotonously with frequency,and the measurement accuracy are higher.Polar cells in biological tissues consumes more electric field energy,which is an important factor for electromagnetic biological effects.

关 键 词：介电谱 电容率 电场能 组织 细胞 溶液 生物效应 

分 类 号：R318[医药卫生—生物医学工程] Q64[医药卫生—基础医学]