基于荧光碳点与纳米片MnO_(2)的内滤效应对谷胱甘肽的检测  

Detection of Glutathione Based on the Internal Filtration Effect of Fluorescent Carbon Dots and MnO_(2)Nanosheets

作　　者：王军才 程良玉 秦莉莉 尚冰冰 Wang Juncai;Chen Liangyu;Qin Lili;Shang Bingbing(Hubei Key Laboratory of Biologic Resources Protection and Utilization,Hubei Minzu University,Enshi 445000,China;School of Chemistry and Environmental Engineering,Hubei Minzu University,Enshi 445000,China)

机构地区：[1]湖北民族大学生物资源保护与利用湖北省重点实验室,湖北恩施445000 [2]湖北民族大学化学与环境工程学院,湖北恩施445000

出　　处：《山东化工》2023年第9期140-144,共5页Shandong Chemical Industry

基　　金：湖北省教育厅科学技术研究项目(B2021157);国家自然科学基金项目(22264014);生物资源保护与利用湖北省重点实验室开放基金项目(PT012106);湖北民族大学博士启动基金项目(MY2015B019)。

摘　　要：以解聚后的Kraft硫酸盐木质素(Depolymerized Kraft Sulfate Lignin,DKL)为原料,通过水热法制备出荧光碳点(Carbon Dots,CDs)。采用透射电子显微镜(Transmission Electron Microscopy,TEM)、Fourier变换红外光谱(Fourier Transform Infrared Spectroscopy,FT-IR)以及荧光分光光度计分别对CDs的粒径、结构、荧光性能进行表征。提出了基于内滤效应(Internal Filtration Effect,IFE)的CDs-MnO_(2)荧光探针用于谷胱甘肽(GSH)的检测,即MnO_(2)纳米片宽的吸收与CDs荧光激发光谱重叠,引起荧光猝灭,导致IFE现象发生。将GSH加入CDs-MnO_(2)荧光体系中,体系的荧光得到恢复。实验结果表明,MnO_(2)纳米片的浓度为25μmol/L时,L-CDs的荧光强度有明显下降趋势,且L-CDs的猝灭效率接近30%,反应时间15 min是最佳反应时间。GSH浓度添加到200μmol/L时,荧光强度恢复均衡。该设计具有制备简单、快速检测等优点,为GSH的检测又提供一种简便方法。Lignin carbon dots(L-CDs)were prepared by hydrothermal method using depolymerized Kraft sulfate lignin(DKL)as raw material.The particle size,structure and fluorescence properties of L-CDs were characterized by transmission electron microscopy(TEM),Fourier transform infrared spectroscopy(FT-IR)and fluorescence spectrophotometer.A L-CDs-MnO_(2)fluorescent probe based on internal filter effect(IFE)was proposed for the detection of glutathione(GSH)activity.Due to the overlap between the wide absorption of MnO_(2)nanosheets and the fluorescence excitation spectrum of L-CDs,the fluorescence quenching occurs,resulting in IFE phenomenon.GSH was added into the L-CDs-MnO_(2)fluorescence system and the fluorescence was restored.The results showed that when the concentration of MnO_(2)nanosheets was 25μmol/L,the fluorescence intensity of L-CDs decreased significantly,and the quenching efficiency of L-CDs was close to 30%.The best reaction time is 15 min.When GSH concentration was added to 200μmol/L,the fluorescence intensity returned to equilibrium.The design has the advantages of simple preparation and rapid detection,and provides a simple method for the detection of GSH.

关 键 词：内滤效应 Lignin碳点 谷胱甘肽 活性检测 

分 类 号：O657.3[理学—分析化学]