防治甜瓜细菌性果斑病种传病原菌的杀菌剂筛选  

Screening of bactericides for controlling seed borne pathogens of melon bacterial fruit blotch

作　　者：林涛[1] 马国斌[1] 姜守阳 张克岩 李菊芬[1] LIN Tao;MA Guobin;JIANG Shouyang;ZHANG Keyan;LI Jufen(Protected Horticultural Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai Key Laboratory of Protected Horticultural Technology,Shanghai 201106,China;Shanghai Agricultural Seed and Seedling Co.,Ltd.,Shanghai 201106,China)

机构地区：[1]上海市农业科学院设施园艺研究所,上海市设施园艺技术重点实验室,上海201106 [2]上海农科种子种苗有限公司,上海201106

出　　处：《上海农业学报》2023年第3期55-60,共6页Acta Agriculturae Shanghai

基　　金：上海市2020年长三角构建区域创新共同体领域项目(20312420400)。

摘　　要：选择16种杀菌剂对甜瓜细菌性果斑病菌进行抑菌环试验,筛选到5种具有不同程度抑菌效果的杀菌剂,并选用其中抑菌效果较好的3种杀菌剂(杀菌剂1号200倍液、萎锈·福美双100倍液和0.3%四霉素250倍液)处理甜瓜带菌种子。结果表明:杀菌剂处理后,PCR检测结果仍为阳性,但在杀菌剂1号200倍液处理的甜瓜带菌种子中未分离到致病活菌,0.3%四霉素250倍液处理后种子的带菌量明显减少,而萎锈·福美双100倍液则无明显杀菌效果。杀菌剂1号200倍液和0.3%四霉素250倍液处理对种子发芽无明显影响,而苗期发病率与对照相比均显著降低。Sixteen kinds of bactericides were selected for the antibacterial ring test on the bacterial fruit blotch pathogen of melon,and 5 kinds of bactericides with varying degrees of antibacterial effects were selected.Among them,3 kinds of bactericides[Shajunji 1(200 times solution),Weixiu·Fumeishuang(100 times solution)and 0.3%tetracycline(250 times solution)]with better antibacterial effects were selected to treat melon seeds with bacteria.The results showed that after being treated with bactericide,the PCR detection results were still positive,but no live bacteria was isolated from the melon seeds treated with Shajunji 1(200 times solution).After being treated with 0.3%tetracycline(250 times solution),the bacteria carrier amount of the seeds was significantly reduced,while the bactericidal effect of Weixi·Fumeishuang(100 times solution)was not significant.Shajunji 1(200 times solution)and 0.3%tetracycline(250 times solution)had no significant effect on seed germination,but the seedling morbidity was significantly lower than that of control.

关 键 词：甜瓜种子 细菌性果斑病 杀菌剂 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]