Trilayer anisotropic structure versus randomly oriented structure in heart valve leaflet tissue engineering  

作　　者：Yuriy Snyder Soumen Jana 

机构地区：[1]Department of Bioengineering,University of Missouri,Columbia,MO 65211,USA

出　　处：《Bio-Design and Manufacturing》2023年第4期423-438,共16页生物设计与制造（英文）

基　　金：supported by the National Institute of Health(No.NIH R00HL134823).

摘　　要：It has been hypothesized that leaflet substrates with a trilayer structure and anisotropicmechanical properties could be useful for the production of functional and long-lasting tissue-engineered leaflets.To investigate the influence of the anisotropic structural and mechanical characteristics of a substrate on cells,in this study,we electrospun trilayer anisotropic fibrous substrates and randomly oriented isotropic fibrous substrates(used as controls)from polycaprolactone polymers.Consequently,the random substrates had higher radial and lower circumferential tensile properties than the trilayer substrates;however,they had similar flexural properties.Porcine valvular interstitial cells cultured on both substrates produced random and trilayer cell-cultured constructs,respectively.The trilayer cell-cultured constructs had more anisotropic mechanical properties,17%higher cellular proliferation,14%more extracellular matrix(i.e.,collagen and glycosaminoglycan)production,and superior gene and protein expression,suggesting that more cells were in a growth state in the trilayer constructs than in the random constructs.Furthermore,the random and radial layers of the trilayer constructs had more vimentin,collagen,transforming growth factor-beta 1(TGF-ß1),transforming growth factor-beta 3(TGF-ß3)gene expression than in the circumferential layer of the constructs.This study verifies that the differences in structural,tensile,and anisotropic properties of the trilayer and random substrates influence the characteristics of the cells and ECM in the constructs.

关 键 词：Heart valve leaflet TRILAYER Tissue engineering Fiber ELECTROSPINNING 

分 类 号：R318.11[医药卫生—生物医学工程]