3D-printed engineered bacteria-laden gelatin/sodium alginate composite hydrogels for biological detection of ionizing radiation  

作　　者：Ziyuan Chen Jintao Shen Meng Wei Wenrui Yan Qiucheng Yan Zhangyu Li Yaqiong Chen Feng Zhang Lina Du Bochuan Yuan Yiguang Jin 

机构地区：[1]Department of Pharmaceutical Sciences,Beijing Institute of Radiation Medicine,Beijing 100850,China

出　　处：《Bio-Design and Manufacturing》2023年第4期439-450,共12页生物设计与制造（英文）

基　　金：supported by the Special Program for Capability Promotion

摘　　要：Nuclear safety is a global growing concern,where ionizing radiation(IR)is a major injury factor resulting in serious damage to organisms.The detection of IR is usually conducted with physical dosimeters;however,biological IR detection methods are deficient.Here,a living composite hydrogel consisting of engineered bacteria and gelatin/sodium alginate was 3D-printed for the biological detection of IR.Three strains of PrecA::egfp gene circuit-containing engineered Escherichia coli were constructed with IR-dependent fluorescence,and the DH5αstrain was finally selected due to its highest radiation response and fluorescence.Engineered bacteria were loaded in a series of gelatin/sodium alginate matrix hydrogels with different rheology,3D printability and bacterial applicability.A high-gelatin-content hydrogel containing 10%gelatin/1.25%sodium alginatewas optimal.The optimal living composite hydrogelwas 3D-printedwith the special bioink,which reported significant green fluorescence underγ-ray radiation.The living composite hydrogel provides a biological strategy for the detection of environmental ionizing radiation.

关 键 词：3D printing ALGINATE Engineered bacteria GELATIN HYDROGEL Ionizing radiation 

分 类 号：R318.08[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]