CO_(2)浓度升高对栎黄枯叶蛾解毒酶和保护酶活性的影响  

Effects of elevated CO_(2) concentration on detoxification enzyme and protective enzyme activities of Trabala vishnou gigantina Yang

作　　者：娄丁 李想 薛喜琴 王盼 刘永华 LOU Ding;LI Xiang;XUE Xi-qin;WANG Pan;LIU Yong-hua(Yulin University/Shaanxi Key Laboratory of Ecological Restoration in Shanbei Mining Area,Yulin 719000,Shaanxi,China)

机构地区：[1]榆林学院/陕西省陕北矿区生态修复重点实验室,陕西榆林719000

出　　处：《湖北农业科学》2023年第6期82-85,151,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31760209);陕西省教育厅重点科研计划项目(21JS045)。

摘　　要：为了更好地了解气候变化下栎黄枯叶蛾(Trabala vishnou gigantina Yang)的为害趋势,测定和分析了正常CO_(2)浓度(375μL/L)和高CO_(2)浓度(750μL/L)下栎黄枯叶蛾体内2种解毒酶[谷胱甘肽-S-转移酶(GST)、乙酰胆碱酯酶(AchE)]和3种保护酶[过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)]的活性。结果表明,栎黄枯叶蛾不同龄期幼虫体内2种解毒酶的活性在高CO_(2)浓度下呈上升趋势,其中GST活性在高CO_(2)浓度下均高于正常CO_(2)浓度,1龄、5龄和6龄幼虫体内的GST活性与正常CO_(2)浓度之间差异显著;在高CO_(2)浓度下,3龄至6龄幼虫的AchE活性均有所升高,其中,5龄、6龄幼虫体内AchE活性显著高于正常CO_(2)浓度下相应龄期的活性。在高CO_(2)浓度下,栎黄枯叶蛾不同龄期幼虫体内3种保护酶随龄期的变化不一致,其中CAT活性均呈上升趋势,且显著高于正常CO_(2)浓度下相应龄期的活性;POD活性随着龄期的增加呈先上升后下降的趋势,1龄、2龄、6龄幼虫体内POD活性变化不显著;SOD活性随龄期的增加整体呈下降趋势,4龄、5龄、6龄栎黄枯叶蛾幼虫体内SOD活性均显著低于正常CO_(2)浓度下相应龄期的活性。To better understand the impact of climate change on Trabala vishnou gigantina Yang,the activities of two detoxification enzymes[glutathione S-transferase(GST),acetylcholinesterase(AchE)]and three protective enzymes[catalase(CAT),peroxidase(POD)and superoxide dismutase(SOD)]were determined and analyzed under normal CO_(2) concentration(375μL/L)and high CO_(2) concentration(750μL/L).The results showed that the activities of the two detoxification enzymes in the larvae of different instars of Trabala vishnou gigantina Yang showed an increasing trend under high CO_(2) concentration,the activity of GST was higher than that un⁃der normal CO_(2) concentration,and the activities of GST in the larvae of the first,fifth and sixth instars were significantly different from those under the normal CO_(2) concentration;under high CO_(2) concentration,AchE activity of the 3rd to 6th instars was increased,and AchE activity of the 5th and 6th instars was significantly higher than that of the corresponding instars under normal CO_(2) concentration.Under high CO_(2) concentration,the changes of the three protective enzymes in the larvae of different instars of Trabala vishnou giganti⁃na Yang were inconsistent with each other,and the activity of CAT showed an increasing trend,which was significantly higher than that of the corresponding instars under normal CO_(2) concentration;the POD activity increased first and then decreased with the increase of instar,and there was no significant change of POD activity in the first instar,second instar and sixth instar larvae;the SOD activity of the larvae decreased with the increase of instars,which was significantly lower in the 4th,5th and 6th instars than that of the corre⁃sponding instars under normal CO_(2) concentration.

关 键 词：栎黄枯叶蛾(Trabala vishnou gigantina Yang) CO_(2)浓度 保护酶 解毒酶 活性 

分 类 号：Q968[生物学—昆虫学]