厚朴中总鞣质提纯工艺及抗菌活性研究  

Study on the purification technology and antibacterial activity of total tannins from Magnolia officinalis

作　　者：杨敏[1] 奚军伟 YANG Min;XI Jun-wei(Shaanxi Institute of International Trade&Commerce,Xi’an 712046,China;Shaanxi Adverse Drug Reaction Monitoring Center,Xi’an 710000,China)

机构地区：[1]陕西国际商贸学院,西安712046 [2]陕西省药品不良反应监测中心,西安710000

出　　处：《湖北农业科学》2023年第6期129-134,共6页Hubei Agricultural Sciences

基　　金：国家科技部重大新药创制项目(2018ZX09721-005-009-013);陕西国际商贸学院中药质量标志物创新团队项目(SSY18TD02)。

摘　　要：通过响应面设计优化厚朴(Magnolia officinalis Rehd.et Wils.)中总鞣质的提取工艺,并进一步纯化。结果表明,厚朴中总鞣质的最佳提取工艺为料液比1∶13、提取时间100 min、乙醇浓度80%、超声功率80 W,厚朴总鞣质提取量为0.1810 mg/g,与理论值0.1888 mg/g差距小。利用正交试验考察明胶溶液沉淀法对厚朴总鞣质的最佳纯化工艺为浓缩液比重1.5 mg/mL、明胶溶液浓度4%、明胶溶液添加量8 mL/100 mg,厚朴总鞣质提取量为2.743 mg/g。利用最小抑菌浓度测定法比较总鞣质对金黄色葡萄球菌和大肠埃希菌的抗菌活性,表明厚朴中总鞣质具有一定的抗菌活性。The extraction process of total tannins from Magnolia officinalis was optimized by response surface design and further puri⁃fied.The results showed that the best extraction process for total tannins from Magnolia officinalis was the ratio of material to liquid of 1∶13,extraction time of 100 min,ethanol concentration of 80%and ultrasonic power of 80 W.The total tannins extracted from Magno⁃lia officinalis was 0.1810 mg/g,which was not far from the theoretical value of 0.1888 mg/g.The optimum purification process of total tannins from Magnolia officinalis by gelatin solution precipitation was studied by an orthogonal test.When the specific gravity of con⁃centrated solution was 1.5 mg/mL,the concentration of gelatin solution was 4%,and the amount of gelatin solution added was 8 mL/100 mg,the total tannins extracted from Magnolia officinalis was 2.743 mg/g.The antibacterial activities of total tannins against Staph⁃ylococcus aureus and Escherichia coli were compared by the minimum inhibitory concentration method.The results showed that the total tannins from Magnolia officinalis had certain antibacterial activity.

关 键 词：厚朴(Magnolia officinalis Rehd.et Wils.) 总鞣质 响应面法 正交试验 抗菌活性 

分 类 号：R284.2[医药卫生—中药学]