基于生物信息学分析探讨子宫内膜异位症恶变的机制  

Exploring the mechanism of malignant transformation of endometriosis based on bioinformatics analysis

作　　者：吴桃 呼学敏 杨玉芳 杜雪 WU Tao;HU Xue-min;YANG Yu-fang;DU Xue(Graduate School,Tianjin Medical University,Tianjin 300070,China;Department of Gynecology,Tianjin Union Medical Center,Nankai University,Tianjin 300121,China)

机构地区：[1]天津医科大学研究生院,天津300070 [2]天津市人民医院/南开大学人民医院妇科,天津300121

出　　处：《天津医科大学学报》2023年第4期366-371,共6页Journal of Tianjin Medical University

摘　　要：目的:通过生物信息学分析探讨子宫内膜异位症(EMs)恶变为卵巢透明细胞癌(OCCC)的致病机制。方法:通过GEO数据库微阵列表达谱数据集GSE57545,分别将卵巢型EMs(OVE)、EMs相关OCCC与正常女性个体的免疫基因进行差异分析,并对差异表达基因进行GO富集和KEGG信号通路分析、构建差异表达基因的PPI网络并筛选Hub基因。结果:OVE与对照组间共筛选出43个差异表达基因,OCCC与对照组间共筛选出96个差异表达基因。相对于正常女性而言,KEGG信号通路富集显示:癌症通路是OVE与OCCC共有的信号通路。GO富集分析结果示:免疫系统过程、免疫反应、调节免疫系统进程等是OVE与OCCC共有的生物学过程;囊泡、质膜部分、细胞表面是OVE与OCCC的差异表达基因富集的共有细胞组分。CAPS3是OVE与OCCC的交集Hub基因。结论:免疫系统在EMs恶变的过程中起重要作用;CAPS3可能是EMs恶变为OCCC的关键靶点之一。Objective:To investigate the pathogenesis of malignant transformation of endometriosis(EMs)into ovarian clear cell carcinoma(OCCC)by bioinformatics analysis.Methods:Through GEO database microarray expression profile dataset GSE57545,the immune genes of OVE,EMs related OCCC and normal female individuals were analyzed differentially.GO enrichment and KEGG signaling pathway analysis were performed for differentially expressed genes,PPI networks of differentially expressed genes were constructed and Hub genes were screened.Results:A total of 43 differentially expressed genes were screened between OVE and control group,and 96 differentially expressed genes were screened between OCCC and control group.Compared with normal female individuals,the enrichment of KEGG signaling pathway indicated that the cancer pathway was a common signaling pathway of OVE and OCCC.GO enrichment analysis results showed that the immune system process,immune response,regulation of immune system process were common biological processes of OVE and OCCC.The vesicles,plasma membrane and cell surface were the common components of OVE and OCCC enriched in DEGs.CAPS3 was the intersection Hub gene of OVE and OCCC.Conclusion:The immune system plays an important role in the malignant transformation of endometriosis;CAPS3 may be one of the key targets for the malignant transformation of EMs into OCCC.

关 键 词：子宫内膜异位症 恶变 卵巢透明细胞癌 细胞焦亡 CASPASE-3 

分 类 号：R71[医药卫生—妇产科学]