国内葡萄白粉病菌对戊唑醇的抗药性研究  

Resistance of Erysiphe necator to Tebuconazole in China

作　　者：高琪 刘梅[1] 谭海芸 王琦 李兴红[1] 张玮[1] GAO Qi;LIU Mei;TAN Haiyun;WANG Qi;LI Xinghong;ZHANG Wei(Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China/Institute of Plant Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;College of Plant Protection,China Agricultural University,Beijing 100193,China)

机构地区：[1]北方果树病虫害绿色防控北京市重点实验室/北京市农林科学院植物保护研究所,北京100097 [2]中国农业大学植物保护学院,北京100193

出　　处：《农学学报》2023年第6期49-54,共6页Journal of Agriculture

基　　金：财政部和农业农村部国家现代农业产业技术体系“树体病害防控岗位”(CARS-29);北京市科技计划课题“设施有机生产绿色生防技术研究与示范”(Z201100008020014)。

摘　　要：为明确国内葡萄白粉病菌(Erysiphe necator)对戊唑醇的抗药性,采用实时荧光定量PCR,基于对葡萄白粉病菌CYP51基因第495位点突变A495T的检测,分析国内5个省份分离获得的134株菌株对戊唑醇的抗药性,利用孢子萌发法测定供试菌株对戊唑醇的敏感性,分析2种方法结果的相关性。结果表明,实时荧光定量PCR方法检测到国内葡萄白粉病菌对戊唑醇已出现抗药性,抗性频率为35.07%,不同地区存在差异。孢子萌发法结果显示,供试菌株对戊唑醇的EC_(50)范围为0.085~280.917μg/mL,均值为24.208μg/mL,不同地区菌株对戊唑醇的敏感性差异较大,EC_(50)最大值和最小值之比为2.5~3304。孢子萌发法与实时荧光定量PCR方法间的检测结果趋势一致并显著相关。To clarify the sensitivity of Erysiphe necator isolates to tebuconazole in China,quantitative real-time PCR(qPCR)was used to analyze the resistance of 134 E.necator isolates collected from 5 grape growing provinces,based on the detection of mutation A495T at locus 495 of the CYP51 gene of E.necator.The spore germination method was used to determine the susceptibility of the test isolates to tebuconazole,and the correlation between the results of the two methods was analyzed.The results showed that E.necator isolates had developed resistance to tebuconazole in China,while the resistance frequency was 35.07%,with differences in different regions.The EC_(50) value of all isolates to tebuconazole determined by spore germination method ranged from 0.085 to 280.917μg/mL and the average EC_(50) value was 24.208μg/mL.The ratio of the lowest and the highest EC_(50) values for the isolates from different regions ranged from 2.5 to 3304,indicating that the sensitivity of the isolates from different regions to tebuconazole varied greatly.The results of tebuconazole sensitivity detection of the spore germination method and qPCR method were consistent and significantly correlated.

关 键 词：葡萄白粉病菌 戊唑醇 抗药性 实时荧光定量PCR 孢子萌发 

分 类 号：S481.4[农业科学—农药学]