早期创伤性膝骨关节炎动物模型构建方案的比较  

Comparison of protocols for constructing animal models of early traumatic knee osteoarthritis

作　　者：刘宇涵 樊渝江 王启光[2] Liu Yuhan;Fan Yujiang;Wang Qiguang(Province-Ministry Collaborative Innovation Center for Regenerative Medicine and Medical Bioresource Development and Application,Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China;Center for Biomaterials,Sichuan University,Chengdu 610064,Sichuan Province,China)

机构地区：[1]广西医科大学再生医学与医用生物资源开发应用省部共建协同创新中心,广西壮族自治区南宁市530021 [2]四川大学生物材料中心,四川省成都市610064

出　　处：《中国组织工程研究》2024年第4期542-549,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(32071353),项目名称:基于单细胞封装的软骨细胞周基质(PCM)仿生构建及其作用机理研究,项目负责人:王启光。

摘　　要：背景:现有骨关节炎的有创造模方法包括前交叉韧带横断手术和前交叉韧带横断联合内侧半月板前角切除手术,前交叉韧带横断术后需要过量运动,而完整切除内侧半月板前角可能造成副损伤并增加造模结果的差异性,对术者手术技巧要求较高。目的:对传统的骨关节炎有创造模手段进行改良和简化,并比较不同方案在无高负荷运动环境下的造模效果。方法:采用随机数字表法将48只SD大鼠分为4组,每组12只:假手术组完全暴露左侧后肢膝关节腔后缝合关节腔及皮肤;横断组、横断+切除组、横断+撕裂组左侧后肢分别进行前交叉韧带横断手术、前交叉韧带横断联合内侧半月板前角切除手术、前交叉韧带横断联合内侧半月板前角撕裂手术。术后4周取膝关节标本,进行大体、X射线片、CT扫描、病理组织切片观察及PCR检测。结果与结论:①大体观察:横断组可见半月板处轻度磨损;横断+撕裂组可见外侧髁关节面严重磨损、内侧髁关节面轻度磨损、半月板严重磨损,内侧半月板全层磨损;横断+切除组可见外侧髁关节面严重磨损、内侧髁关节面轻度磨损、半月板内侧前角缺如,半月板磨损面积>50%;②X射线片与CT扫描:4组无明显差异,其中3个手术组可见胫骨前移,横断+切除组内侧半月板前角缺失;③病理组织切片观察:苏木精-伊红、甲苯胺蓝、天狼猩红染色显示,假手术组与横断组关节面光滑平整;横断+撕裂组、横断+切除组可见明显的软骨损伤及基质降解,其中横断+撕裂组软骨损伤及基质降解情况较轻;④PCR检测:横断+撕裂组、横断+切除组白细胞介素1β、白细胞介素6、白细胞介素8、肿瘤坏死因子α、基质金属蛋白酶1、基质金属蛋白酶3的mRNA表达量高于假手术组、横断组(P<0.05),聚集蛋白聚糖的mRNA表达量低于假手术组、横断组(P<0.05);横断+切除组白细胞介素6、基质金属蛋白�BACKGROUND:Current osteoarthritis modeling methods include anterior cruciate ligament transection(ACLT)and ACLT combined with medial meniscal anterior horn resection.ACLT requires excessive postoperative exercise,which is time and labor-intensive.Complete removal of anterior horn of the medial meniscus can cause collateral damage and increase variability in modeling outcomes,requiring higher surgical skills from the surgeon.OBJECTIVE:To modify and simplify the traditional method to create animal osteoarthritis model and compare osteoarthritis symptoms of different modeling methods under a low-load exercise environment.METHODS:Forty-eight Sprague-Dawley rats were randomly assigned in four groups(n=12 per group):sham operation(complete exposure of the knee cavity of the left hind limb followed by suturing the joint cavity and skin),ACLT,ACLT+anterior horn resection(removal of the anterior horn of the medial meniscus)and ACLT+anterior horn tear(anterior horn tear of the medial meniscus).At 4 weeks after modeling,the rats were euthanized and their knee specimens were collected for gross observation,X-ray and CT scans,pathological observation,and PCR detection.RESULTS AND CONCLUSION:Gross observation:Mild meniscal wear was observed in the ACLT group.In the ACLT+anterior horn tear group,severe wear of the lateral condyle articular surface,mild wear of the medial condyle articular surface,severe meniscal wear,and full wear of the medial meniscus were observed.The ACLT+resection group showed severe wear of the lateral condyle articular surface,mild wear of the medial condyle articular surface,absence of the anterior horn of the medial meniscus,and meniscus wear area>50%.Imaging examinations showed no significant difference among the four groups.However,the anterior tibial translocation sign was observed in the three operation groups and the anterior horn of the medial meniscus was missing in the ACLT+anterior horn resection group.Histopathological section observation:Hematoxylin-eosin,toluidine blue,and Sirius red staini

关 键 词：骨关节炎 前交叉韧带横断术 内侧半月板失稳术 创伤后骨关节炎 实验造模 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R365