三七总皂苷上调浓缩生长因子释放促进大鼠骨折愈合  

Panax notoginseng saponin promotes fracture healing by upregulating concentrated growth factors in rats

作　　者：王钟庆 熊贤梅 张严 李世杰 马立琼 卢泽声 高怡加[2] Wang Zhongqing;Xiong Xianmei;Zhang Yan;Li Shijie;Ma Liqiong;Lu Zesheng;Gao Yijia(First Clinical School of Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China)

机构地区：[1]广州中医药大学第一临床医学院,广东省广州市510405 [2]广州中医药大学第一附属医院,广东省广州市510405

出　　处：《中国组织工程研究》2024年第11期1678-1683,共6页Chinese Journal of Tissue Engineering Research

基　　金：广东省自然科学基金项目(2018A030313369),项目负责人:高怡加;国家自然科学基金资助项目(81974575),项目参与人:张严;黄枫全国名老中医药专家传承工作室建设项目({2022}75号),项目参与人:高怡加;2022广州中医药大学“双一流”与高水平大学建设学科后备人才培育项目专项经费。

摘　　要：背景:研究表明,三七总皂苷和浓缩生长因子均能促进骨折愈合,但是目前关于两者共同干预骨折愈合的研究较少,三七总皂苷可能通过在某段时间内促进浓缩生长因子相关因子的释放来达到加快骨折愈合的目的。目的:观察三七总皂苷对浓缩生长因子释放,以及对大鼠骨折愈合的影响。方法:8周龄SD大鼠18只,编号后随机分为三七总皂苷组、模型对照组以及空白组。三七总皂苷组给予三七总皂苷灌胃2周,模型对照组给予生理盐水2 mL灌胃2周,空白组正常饲喂。2周后三七总皂苷组与模型对照组同时采血离心获取浓缩生长因子备用,继续正常饲喂1周,然后对所有大鼠进行股骨骨折造模处理,三七总皂苷组与模型对照组植入自体浓缩生长因子,并用ELISA法检测1 h及1,3,5,7,9,11 d生长因子释放量;通过观察术后2个月X射线片及骨组织苏木精-伊红染色结果,评估骨折愈合情况。结果与结论:①生长因子释放情况:三七总皂苷组的血管内皮生长因子A和转化生长因子β在第7,9,11天的释放浓度高于模型对照组(P<0.05),血小板衍生生长因子BB在第5,9,11天的释放浓度高于模型对照组(P<0.05),碱性成纤维细胞生长因子在1-11天的释放浓度均高于模型对照组(P<0.05);②骨折愈合情况:术后2个月行X射线片检查,三七总皂苷组的骨折愈合情况优于模型对照组,两组均优于空白组;取材苏木精-伊红染色发现,三七总皂苷组成骨细胞生成量大于模型对照组,两组均大于空白组;③提示三七总皂苷可以上调浓缩生长因子相关因子的释放浓度,三七总皂苷干预后的浓缩生长因子对促进大鼠骨折愈合更有优势。BACKGROUND:Studies have shown that both Panax notoginseng saponins and concentrated growth factor can promote fracture healing,but there are few studies addressing their combined effects on fracture healing.Panax notoginseng saponins may accelerate fracture healing by promoting the release of concentrated growth factor-related factors over a certain period of time.OBJECTIVE:To study the effect of Panax notoginseng saponins on concentrated growth factor release and fracture healing in rats.METHODS:Eighteen 8-week-old Sprague-Dawley rats were numbered and randomly divided into three groups:Panax notoginseng saponins group,model control group and blank group.Panax notoginseng saponins group was fed with Panax notoginseng saponins for 2 weeks.Model control group was given 2 mL of normal saline for 2 weeks and blank group was fed normally.Concentrated growth factor was obtained by the centrifugation method both from the Panax notoginseng saponins group and model control group.After 1 week of normal feeding,all animals underwent modeling for femoral fracture.The Panax notoginseng saponins group and the model control group were implanted with autologous concentrated growth factor,and then the release concentration of growth factors at different time points(1 hour,1,3,5,7,9 and 11 days)were measured by ELISA.Fracture healing was assessed based on postoperative X-ray and hematoxylin-eosin staining of bone tissues.RESULTS AND CONCLUSION:Compared with the model control group,the Panax notoginseng saponins group had higher release concentrations of vascular endothelial growth factor A and transforming growth factorβat 7,9,and 11 days,Platelet-derived growth factor BB at 5,9,and 11 days,and basic fibroblast growth factor at 1-11 days(P<0.01).X-ray examinations indicated that fracture healing in the Panax notoginseng saponins group was better than that in the model control group,and fracture healing in these two groups was better than that in the blank group at 2 months after surgery.Hematoxylin-eosin staining results found t

关 键 词：三七总皂苷 浓缩生长因子 骨折愈合 大鼠 动物模型 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R496