水稻热激转录因子家族基因对纹枯病病菌和植物激素的响应特征  

Response characteristics of rice heat shock factors family genes to Rhizoctonia solani and plant hormones

作　　者：高鹏 王广达 魏兆根 叶元妹 冯志明 赵剑华 居冉 谢文亚 陈宗祥[1,2] 胡珂鸣 左示敏 GAO Peng;WANG Guang-da;WEI Zhao-gen;YE Yuan-mei;FENG Zhi-ming;ZHAO Jian-hua;JU Ran;XIE Wen-ya;CHEN Zong-xiang;HU Ke-ming;ZUO Shi-min(Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of Ministry of Education/College of Agriculture,Yangzhou University,Yangzhou 225009,China;Jiangsu Collaborative Innovation Center for Modern Industrial Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology/Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学农学院/江苏省作物基因组学和分子育种重点实验室/植物功能基因组学教育部重点实验室,江苏扬州225009 [2]扬州大学/江苏省粮食作物现代产业技术协同创新中心/江苏省作物遗传生理重点实验室,江苏扬州225009

出　　处：《江苏农业学报》2023年第3期609-621,共13页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31872858、32000362);江苏省政府种业振兴项目[JBGS(2021)001];江苏省自然科学基金资助项目(BK20200930);江苏省研究生科研创新计划资助项目(KYCX20_2985);扬州大学“青蓝工程”项目。

摘　　要：解析水稻热激转录因子(Heat shock factors,Hsf)家族基因响应纹枯病病菌侵染和4种植物激素处理的表达特征,可为进一步解析Hsf调控水稻纹枯病抗性与对相关逆境的响应提供重要依据。利用生物信息学方法搜索并鉴定到25个水稻Hsf基因,对其系统进化树、相关分子特征、蛋白质结构域、基因结构及顺式作用元件进行预测和分析,用荧光定量PCR法分析它们对纹枯病病菌侵染的响应特征及4种激素[茉莉酸(Jasmonic acid,JA)、水杨酸(Salicylic acid,SA)、乙烯(Ethylene,ETH)和激动素(Kinetin,KT)]处理后的表达模式,同时分析它们在水稻组织中的表达情况。结果显示,水稻Hsf蛋白总体上可划分为5组,3个OsHsf亚家族基因在进化关系上的距离较远。25个Hsf蛋白的相对分子质量大小不一,且多数蛋白质的稳定性不高,但其基因结构较为保守。这些基因可能与激素响应及光信号通路相关。有5个基因呈组成型表达,另有7个基因呈组织特异性表达。水稻Hsf基因总体上受纹枯病病菌诱导的强度较低,其中11个上调表达的基因主要集中在OsHsfA、OsHsfB亚家族,4个下调表达的基因主要集中在OsHsfC亚家族,提示Hsf基因可能在调控水稻对纹枯病抗性的功能上存在分化。4个基因(OsHsfA2a、OsHsfA3、OsHsfB2a、OsHsfB2c)强烈响应纹枯病病菌的侵染,并且在叶鞘、叶片组织中的相对表达量较高,表明这些基因可能参与调控水稻对纹枯病的抗性。多数Hsf基因能够响应4种植物激素处理,总体来说,大部分OsHsf基因在JA、SA和ETH处理下呈下调表达,仅有少数基因呈上调表达;JA处理与SA处理相比,有1个Hsf基因受诱导表达的特征相反,SA处理和ETH处理相比,有3个Hsf基因受诱导表达的特征相反,JA处理和ETH处理相比,有3个Hsf基因受诱导表达的特征相反,JA处理与SA处理相比,SA处理与ETH处理相比,JA处理与ETH处理相比,表达特征相似的基因分别有18个、1Characterization of the expression of rice heat shock factors(Hsf)family genes in response to the infection of Rhizoctonia solani and the treatment of four plant hormones can provide an important basis for further analysis of the regulation of sheath blight resistance and related stress response by Hsf.Twenty-five rice Hsf genes were identified by searching using bioinformatics methods,and their phylogenetic tree,molecular characteristics,protein domains,gene structure and cis-acting elements were predicted and analyzed,while the response characteristics to the infection of R.solani and the expression patterns of four plant hormones(jasmonic acid:JA,salicylic acid:SA,ethylene:ETH,kinetin:KT)after treatment,and the expression patterns in different tissues in rice were analyzed through qPCR.Overall,the rice Hsf proteins could be classified into five groups.The three OsHsf subfamily genes were evolutionarily distant from each other.The 25 Hsf proteins had different molecular weights and most of them were unstable,but their gene structures were relatively conserved.These genes might be associated with hormone response as well as light signaling pathways.Five genes were constitutively expressed and seven genes were tissue-specific.In general,rice Hsf genes were less induced by R.solani,with 11 up-regulated genes mainly in the OsHsfA and OsHsfB subfamiliy and four down-regulated genes mainly in the OsHsfC subfamily,suggesting a possible divergence in the evolution of Hsf genes in regulating resistance to sheath blight.Four genes(OsHsfA2a,OsHsfA3,OsHsfB2a and OsHsfB2c)were highly expressed mainly in leaves and leaf sheaths while strongly responding to sheath blight,suggesting that these genes might be involved in regulating resistance to sheath blight.Most Hsf genes were able to respond to the treatments of four plant hormones,and overall,most OsHsf genes showed down-regulated expression in JA,SA and ETH treatments,and only a few showed up-regulated expression.There were one,three and three Hsf with opposite induced exp

关 键 词：水稻 OsHsf基因 纹枯病 防御相关激素 

分 类 号：S435.111.42[农业科学—农业昆虫与害虫防治]