藜麦SSR指纹图谱构建及遗传多样性分析  

Fingerprint Construction and Genetic Diversity Analysis of Quinoa Based on SSR Markers

作　　者：陈翠萍[1,2] 闫殿海 张书苗 左皓南 高森 刘洋 Chen Cuiping;Yan Dianhai;Zhang Shumiao;Zuo Haonan;Gao Sen;Liu Yang(Academy of Agricultural and Forestry Sciences,Qinghai University,Xining 810016,Qinghai,China;Xining Sub-Center for New Plant Variety Tests,Ministry of Agriculture and Rural Affairs,Xining 810016,Qinghai,China)

机构地区：[1]青海大学农林科学院,青海西宁810016 [2]农业农村部植物新品种测试(西宁)分中心,青海西宁810016

出　　处：《作物杂志》2023年第3期35-42,共8页Crops

基　　金：农业农村部品种资源保护项目(2020年)。

摘　　要：利用前期筛选出的18对SSR引物对96份不同来源的藜麦种质材料进行遗传多样性分析,构建DNA指纹数据库。结果表明,筛选的18对SSR引物共检测到94个等位基因位点,每对引物检测出3~10个等位基因基因位点;利用18对引物组合可将96份藜麦种质材料鉴定出来;96份藜麦遗传相似系数变幅为0.55~0.98,平均遗传相似系数为0.77;聚类结果将96份藜麦材料分为三大类。The total of 18 pairs of SSR primers were selected to analyze the genetic diversity of 96 quinoa germplasm materials from different sources,and a DNA fingerprint database was constructed.The results showed that 94 alleles were detected by 18 pairs of SSR primers,and 3-10 alleles were detected each pair of SSR primers.Using 18 pairs of primers,96 quinoa germplasm materials could be identified.The genetic similarity coefficients varied from 0.55 to 0.98,with an average of 0.77.The 96 quinoa materials were classified into three categories.

关 键 词：藜麦 SSR标记 指纹图谱 遗传多样性 

分 类 号：S519[农业科学—作物学]