玉米―大刍草渗入系群体的剑叶遗传基础解析  

Dissecting the Genetic Basis of Flag Leaf in Maize-Teosinte Introgression Line Population

作　　者：高沐甜 邱冠杰 朱通通 李瑞莲[1,2,3] 邓敏 罗红兵 黄成[1,2,3] Gao Mutian;Qiu Guanjie;Zhu Tongtong;Li Ruilian;Deng Min;Luo Hongbing;Huang Cheng(College of Agronomy,Hunan Agricultural University,Changsha 410128,Hunan,China;Key Laboratory of the Ministry of Education for Crop Physiology and Molecular Biology,Changsha 410128,Hunan,China;Maize Engineering Technology Research Center of Hunan Province,Changsha 410128,Hunan,China)

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]作物生理与分子生物学教育部重点实验室,湖南长沙410128 [3]湖南省玉米工程技术研究中心,湖南长沙410128

出　　处：《作物杂志》2023年第3期51-57,共7页Crops

基　　金：长沙市杰出创新青年培养计划项目(kq2209016);湖南省普通高校青年骨干教师培养项目;湖南省作物种质创新与资源利用重点实验室开放研究项目(19KFXM10);湖南农业大学青年科学基金项目(19QN16)。

摘　　要：玉米剑叶是玉米雌穗苞叶顶端的延伸,其不仅受气候和土壤等环境因素影响,同时也受遗传因素控制。利用一套由玉米自交系W22与玉米野生祖先种大刍草(Zea mays ssp.parviglumis)杂交衍生得到的包含866个家系的渗入系材料,结合19838个SNP分子标记,采用R/qtl的多QTL模型对玉米剑叶进行QTL定位分析。结果显示,共检测到9个控制玉米剑叶的QTL,分别位于第1、2、3、4、5、7和8号染色体。9个QTL共解释30.94%的表型变异,单个QTL的表型贡献率变幅为1.76%~11.51%,加性效应的变幅为-0.03~0.12,表明玉米剑叶是由多基因控制的数量性状。相关性分析表明,玉米剑叶与株高、穗位高、茎长和叶绿素含量呈显著正相关,与百粒重、粒宽和粒厚呈显著负相关。进一步利用转录组数据对最大效应QTL(qFL3-1)进行候选基因表达分析,筛选出5个可能的候选基因。Maize flag leaves are extended from the tips of ear husks,which are not only affected by environmental factors such as climate and soil,but also controlled by genetic factors.Using a large population of 866 maize-teosinte introgression lines that had been genotyped with 19838 SNP markers,we performed a QTL mapping for maize flag leaf by applying the multiple QTL model in R/qtl.The results showed that a total of nine QTLs for maize flag leaf were located on chromosome 1,2,3,4,5,7 and 8.All nine loci attributed to 30.94%of the phenotypic variation,with the phenotypic contribution rate and additive effect of each QTL ranged from 1.76%to 11.51%and from-0.03 to 0.12,respectively,indicating that the maize flag leaf was a quantitative trait controlled by a number of loci.Pearson correlation coefficient analysis showed that maize flag leaf was significantly positively correlated with plant height,ear height,stem length,and chlorophyll content,and it was significantly negatively correlated with 100-seed weight,seed width,and seed thickness.Further analysis of candidate genes for the largest effect QTL(qFL3-1)using the transcriptome data,five possible candidate genes were screened out.

关 键 词：玉米 大刍草 渗入系群体 剑叶 遗传基础 

分 类 号：S513[农业科学—作物学]