水杨酸对盐胁迫下棉苗生长及生理的调控作用  

Effects of Salicylic Acid Application on the Growth and Physiological Characteristics of Cotton Seedlings under Salt Stress

作　　者：徐雪雯 王兴鹏[1,2,3] 王洪博 李国辉 唐茂淞 曹振玺 Xu Xuewen;Wang Xingpeng;Wang Hongbo;Li Guohui;Tang Maosong;Cao Zhenxi(College of Water Resources and Construction Engineering,Tarim University,Alar 843300,Xinjiang,China;Key Laboratory of Modern Agricultural Engineering,Tarim University,Alar 843300,Xinjiang,China;Key Laboratory of Water-Saving Agriculture in Northwest Oasis,Ministry of Agriculture and Rural Affairs,Shihezi 832000,Xinjiang,China)

机构地区：[1]塔里木大学水利与建筑工程学院,新疆阿拉尔843300 [2]塔里木大学现代农业工程重点实验室,新疆阿拉尔843300 [3]农业农村部西北绿洲节水农业重点实验室,新疆石河子832000

出　　处：《作物杂志》2023年第3期188-194,共7页Crops

基　　金：塔里木大学校长基金(TDZKSS202248);国家重点研发计划子课题(2022YFD1900505);兵团财政科技计划项目(2021AA003)。

摘　　要：为探究水杨酸(salicylic acid,SA)对盐胁迫下棉花幼苗生长及生理的调控作用,设置3(低盐,T_(1))、6g/L(高盐,T_(2))2个盐分浓度和0.00(S_(0))、0.01(低浓度,S_(1))、0.05(中浓度,S_(2))、0.10mmol/L(高浓度,S3)4个SA浓度,无SA无盐为对照(CK),基于主成分综合评价法研究SA对盐胁迫下棉花幼苗根系干物质量、根系形态、抗氧化酶及渗透调节物质的影响。主成分分析表明,棉花幼苗根表面积、地下部干物质、根体积和过氧化氢酶(CAT)活性对盐分胁迫及SA的调控响应度最高,盐浓度相同时,随SA浓度的增加呈先增加后减小的趋势,T_(1)S_(2)处理以上4个指标较T_(1)S_(0)分别增加66%、75%、119%和26%;T_(2)S_(2)处理较T_(2)S_(0)分别增加47%、61%、49%和12%。随着盐浓度的增加,根表面积、地下部干物质、根体积呈显著降低趋势,T_(2)S_(0)处理较T_(1)S_(0)分别降低33%、25%和22%,而CAT活性随着盐浓度的增加呈显著增加趋势,T_(2)S_(0)处理较T_(1)S_(0)处理增加32%。综合评价表明,中浓度(0.05mmol/L)SA缓解了低盐胁迫对棉花幼苗的损害,随盐浓度的提高,SA的缓解作用减弱,本研究可为棉花耐盐抗逆种植提供较为适宜的外源施用策略。To investigate the regulatory effects of salicylic acid(SA)on the growth and physiology of cotton seedlings under salt stress,two salt concentrations of 3(low salt,T_(1))and 6g/L(high salt,T_(2))and four SA concentrations of 0.00(S_(0)),0.01(low concentration,S_(1)),0.05(medium concentration,S_(2))and 0.10mmol/L(high concentration,S3)were set,and no SA,no NaCl was as the control(CK),and the effects of SA on root dry matter mass,root morphology,antioxidant enzymes and osmoregulatory substances of cotton seedlings under salt stress were investigated based on a comprehensive evaluation method of principal components.The effects of SA on root dry matter,root morphology,antioxidant enzymes and osmoregulatory substances of cotton seedlings under salt stress were investigated based on a comprehensive evaluation method.Principal component analysis showed that cotton seedling root surface area,underground dry matter,root volume and catalase(CAT)activity were the most responsive to salt stress and SA regulation,and showed a trend of increasing and then decreasing with increasing SA concentration at the same salt concentration,with T_(1)S_(2) treatment increasing 66%,75%,119%and 26%compared to T_(1)S_(0),respectively;T_(2)S_(2) treatment increasing 47%,61%,49%and 12%compared to T_(2)S_(0),respectively.With increasing salt concentration,root surface area,underground dry matter,and root volume showed a significant decrease,compared with T_(1)S_(0),T_(2)S_(0) treatment decreasing 33%,25%and 22%,respectively,while CAT activity showed a significant increase with the increasing of salt concentration,T_(2)S_(0) treatment increasing 32%compared with T_(1)S_(0) treatment.The comprehensive evaluation showed that the medium concentration of SA(0.05mmol/L)alleviated the damage of low salt stress on cotton seedlings,and the alleviating effect of SA decreased with the increasing of salt concentration.The results could provide a more suitable exogenous application strategy for salt-tolerant and stress-resistant planting of cotton.

关 键 词：盐胁迫 水杨酸 根系形态指标 抗氧化酶 渗透调节物质 综合评价 

分 类 号：S562[农业科学—作物学]