稻瘟病菌类SRRM1转录因子的功能分析  

Functional Analysis of SRRM1-Like Transcription Factor of Magnaporthe grisea

作　　者：邱凯华 方淑梅[1] 梁喜龙[1] Qiu Kaihua;Fang Shumei;Liang Xilong(Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang,China)

机构地区：[1]黑龙江八一农垦大学,黑龙江大庆163319

出　　处：《作物杂志》2023年第3期246-253,共8页Crops

基　　金：黑龙江省自然科学基金(C2016047);黑龙江八一农垦大学研究生创新科研项目(YJSCX2019-Y14);校内培育课题(XZR2014-01)。

摘　　要：转录因子在稻瘟病菌(Magnaporthe grisea)的生长发育、逆境适应以及致病性中发挥着重要作用。为明确类SRRM1转录因子调控的pre-mRNA剪接与稻瘟病菌生长发育和逆境适应的关系,采用以基因重组原理为基础的基因定点敲除技术获取类SRRM1基因缺失菌株,并分析营养生长及逆境适应。结果表明,本研究成功构建敲除载体并敲除了稻瘟病菌Y34菌株中的类SRRM1基因,获得基因缺失突变体。稻瘟病菌中类SRRM1基因缺失未明显影响其营养生长,但对刚果红、十二烷基硫酸钠、山梨醇、Na Cl及杀菌剂吡唑醚菌酯逆境胁迫敏感,表现为抗逆性减弱。类SRRM1在稻瘟病菌逆境适应过程中发挥重要作用。Transcription factors played an important role in the growth and development,adversity adaptation and pathogenicity of Magnaporthe grisea.To clarify the relationship between pre-mRNA splicing regulated by transcription factor SRRM1-like and the growth and adversity adaptation of M.grisea,the SRRM1-like genedeficient strains were obtained by gene-specific knockout technology based on the principle of gene recombination,and analyzed for vegetative growth and adversity adaptation.The results showed that the knockout vector was successfully constructed and the SRRM1-like gene in the rice blast fungus Y34 strain was knocked out,and three gene deletion mutants were obtained.The deletion of the SRRM1-like gene did not significantly affect the biotrophic growth of M.grisea,but it was sensitive to stress by Congo-Red,SDS,sorbitol,NaCl and the fungicide pyraclostrobin,and showed weakened stress resistance.SRRM1-like played an important role in the adversity adaptation of M.grisea.

关 键 词：稻瘟病菌 剪接 PWI结构域 生物信息学分析 基因敲除 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]