丹栀调脂汤对高脂诱导MAFLD大鼠PI3K/AKT/FOXO1信号通路的影响  

Effect of Danzhi Tiaozhi Decoction on the PI3K/AKT/FOXO1 Signaling Pathway in High-Fat Induced MAFLD Rats

作　　者：刘玉玉 隋淼[2] 蒋小飞[2] 汤楠楠 王智明[2] LIU Yu-yu;SUI Miao;JIANG Xiao-fei;TANG Nan-nan;WANG Zhi-ming(Graduate School of Anhui University of Traditional Chinese Medicine,Hefei 230000,China;Xuzhou Affiliated Hospital of Nanjing University of Chinese Medicine,Xuzhou 221000,China)

机构地区：[1]安徽中医药大学研究生院,安徽合肥230000 [2]南京中医药大学徐州附属医院,江苏徐州221000

出　　处：《南京中医药大学学报》2023年第6期541-547,共7页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省中医药管理局面上项目(MS2021048);徐州市医学重点专科建设及高层次人才引进培养项目(2019GG008)。

摘　　要：目的研究丹栀调脂汤对高脂饮食诱导的代谢相关脂肪性肝病(MAFLD)大鼠PI3K/AKT/FOXO1信号通路的影响,探讨其在MAFLD治疗中的作用。方法雄性SD大鼠分为正常组、模型组及丹栀调脂汤高、中、低剂量组。正常组和模型组予等体积的生理盐水,丹栀调脂汤高、中、低剂量组分别予丹栀调脂汤20.4、10.2、5.1 g·kg^(-1)·d^(-1),连续灌胃8周后取材。检测5组大鼠体质量、血脂、血糖、胰岛素水平及HOMA-IR变化情况;肝脏组织脂质沉积情况;Western blot检测PI3K/AKT信号通路中各蛋白表达水平。结果与正常组比较,模型组大鼠体质量、TG、TC、FFA、GLU、INS、LDL-C及HOMA-IR水平明显升高,HDL-C水平明显降低;肝脏组织油红O染色可见明显肿大的肝细胞及明显的脂质沉积;PI3K、AKT蛋白磷酸化表达水平显著降低。相较于模型组,丹栀调脂汤高、中、低剂量组体质量、TG、TC、FFA、GLU、INS、LDL-C及HOMA-IR水平不同程度降低;肝细胞肿大、肝细胞脂滴及排列结构均有不同程度的改善;PI3K、AKT、FOXO1蛋白磷酸化表达水平升高。结论丹栀调脂汤可能通过PI3K/AKT/FOXO1信号通路改善胰岛素抵抗,进而达到防治MAFLD的目的。OBJECTIVE To study the effect of Danzhi Tiaozhi Decoction on the PI3K/AKT/FOXO1 signaling pathway in rats with high-fat-diet(HFD)induced metabolic associated fatty liver disease(MAFLD),and to explore its role in the treatment of MAFLD.METHODS Male SD rats were divided into the normal group,HFD model group,and high,medium,and low dose groups of Danzhi Tiaozhi Decoction.The normal group and HFD group were given equal volumes of saline,while the high,medium,and low dose groups of Danzhi Tiaozhi Decoction were given Danzhi Tiaozhi Decoction 20.4,10.2,and 5.1 g·kg^(-1)·d^(-1),respectively.The samples were collected after 8 weeks of continuous gavage.Detect the changes in body mass,blood lipids,blood glucose,insulin levels,HOMA-IR,and liver tissue lipid deposition in the five groups of rats.Western blot was used to detect the expression levels of proteins in the PI3K/AKT signaling pathway.RESULTS Compared with the normal group,the body mass,TG,TC,FFA,GLU,INS,LDL-C,and HOMA-IR levels of the HFD model group rats were significantly increased,while the HDL-C levels were significantly reduced;oil red O staining of liver tissue reveals obvious enlargement of liver cells and significant lipid deposition;the phosphorylation expression levels of PI3K and AKT proteins were significantly reduced.Compared with the HFD group,the body mass,TG,TC,FFA,GLU,INS,LDL-C,and HOMA-IR levels of the high,medium,and low dose groups of Danzhi Tiaozhi Decoction were significantly declined;the enlarged liver cells,lipid droplets and arrangement structure of liver cells were improved to varying degrees;the phosphorylation expression levels of PI3K and AKT proteins were increased.CONCLUSION Danzhi Tiaozhi Decoction may improve insulin resistance through the PI3K/AKT/FOXO1 signaling pathway,thereby achieving the goal of preventing and treating MAFLD.

关 键 词：丹栀调脂汤 代谢相关脂肪性肝病 PI3K/AKT/FOXO1信号通路 

分 类 号：R285.5[医药卫生—中药学]