梨多倍体种质资源的鉴定  

Identification of pear polyploid germplasm resources

作　　者：齐丹[1] 田路明[1] 董星光[1] 张莹[1] 霍宏亮[1] 徐家玉 刘超 张小双[1] 詹俊宇 张思梦 曹玉芬[1] QI Dan;TIAN Luming;DONG Xingguang;ZHANG Ying;HUO Hongliang;XU Jiayu;LIU Chao;ZHANG Xiaoshuang;ZHAN Junyu;ZHANG Simeng;CAO Yufen(Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Key Laboratory of Horticultural Crops Germplasm Resources Utilization,Ministry of Agriculture and Rural Affairs,Xingcheng,Liaoning 125100)

机构地区：[1]中国农业科学院果树研究所,农业农村部园艺作物种质资源利用重点实验室,辽宁兴城125100

出　　处：《中国果树》2023年第6期10-15,共6页China Fruits

基　　金：中国农业科学院科技创新工程(CAAS-ASTIP-2022-RIP);财政部和农业农村部:国家现代农业产业技术体系建设专项(CARS-28)。

摘　　要：使用SSR荧光分子标记技术与流式细胞术相结合的方法,对保存于国家果树种质兴城梨、苹果圃内的100份梨种质资源进行倍性鉴定。结果表明:共鉴定出18份新的梨多倍体种质,其中三倍体梨种质17份,二、三倍体嵌合体1份;4份存在争议的三倍体种质,分别是其力阿木提、骚梨、六月鲜、窝窝。少量SSR位点的多等位基因扩增不能作为梨多倍体种质资源的判断依据,多个SSR位点的3个等位基因扩增,并结合流式细胞术可以准确鉴别梨多倍体种质资源。In this study,SSR fluorescence molecular marker technology and flow cytometry were used to identify the ploidy of 100 pear germplasm resources preserved in National Repository of Pear and Apple Germplasm Resources(Xingcheng,Liaoning).A total of 18 new polyploid pear germplasms were identified,including 17 triploid pear germplasms and 1 diploid and triploid chimera;4 controversial triploid germplasms,namely‘Qiliamuti’‘Saoli’‘Liuyuexian’and‘Wowo’.The multi-allele amplification of a few number of SSR loci cannot be used as a basis for the identification of pear polyploid germplasm resources.The amplification of three alleles of multiple SSR loci combined with flow cytometry can accurately identify pear polyploid germplasm resources.

关 键 词：梨种质资源 SSR荧光标记 流式细胞术 

分 类 号：S661.2[农业科学—果树学]