硒对大鼠胚胎神经细胞拟老化反应的影响  

Effects of Selenium on Mimetic Aging Actions of Cerebral Neurons of Fetal Rats

作　　者：高良才[1] 陈婉蓉[1] 邬雄飞[1] 沈纪益[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《中国老年学杂志》2002年第6期503-504,共2页Chinese Journal of Gerontology

摘　　要：目的　探讨硒延缓神经细胞衰老的作用机制。方法　以原代培养的 SD大鼠胚胎中脑皮层神经细胞为研究对象 ,从形态和生化方面研究无血清培养对神经细胞的损伤以及亚硒酸钠 (Na2 Se O3)的保护作用。结果　神经细胞在无血清培养条件下 ,细胞中丙二醛 (MDA)含量明显增加 ,超氧化物歧化酶 (SOD)和谷胱甘肽过氧化物酶 (GSH- Px)活力显著下降。扫描电镜观察到细胞膜严重皱缩破裂。细胞培养液中加入一定浓度硒可有效地减轻无血清培养引起的细胞损伤。结论　一定浓度的硒可以通过抗氧化作用来延缓神经细胞衰老。Objective To investigate the delaying aging mechanism of selenium on neurons.Methods using the cultured cerebral cortex neurons of SD fetal rats, the injury induced by serum free cultures and the protection of Na 2SeO 3 were studied.Results When cultured neurons were incubated with serum free cultures, MDA content significantly increased while the activities of SOD and GSH Px decreased, and neuron membrane damages were observed using scanning electron microscope. Selemum with given concentrations into cell cultures could effectively reduce the damages caused by serum free culture. Conclusions Attributing to its anti oxidation, a suitable concentration selenium can delay the aging of neurons

关 键 词：硒 神经细胞 无血清培养 衰老 自由基 

分 类 号：R339.38[医药卫生—人体生理学]