4-苯基丁酸对高糖诱导的人脐静脉内皮细胞损伤的保护作用  

Protective effect of 4-phenyl butyric acid on endothelial cells injury induced by high glucose

作　　者：刘赞朝[1] 孙静华 李洋 赵维丽[1] 李蓉 刘立朋 LIU Zanchao;SUN Jinghua;LI Yang;ZHAO Weili;LI Rong;LIU Lipeng(Hebei Provincial Key Laboratory of Basic Medicine for Diabetes,Shijiazhuang Municipal Second Hospital,Shijiazhuang,Hebei 050000,China;Department of Infection Control,Shijiazhuang Municipal Second Hospital,Shijiazhuang,Hebei 050000,China)

机构地区：[1]河北省石家庄市第二医院河北省糖尿病基础医学研究重点实验室,050000 [2]河北省石家庄市第二医院感控科,050000

出　　处：《重庆医学》2023年第13期1938-1942,共5页Chongqing medicine

基　　金：河北省医学科学研究计划基金项目(20220197);河北省重点研究计划生物医药专项(19277739D)。

摘　　要：目的探究4-苯基丁酸(4-PBA)通过调节过氧化物酶体增殖物激活受体γ共激活因子1α(PGC-1α)对高糖诱导的人脐静脉内皮细胞(HUVECs)损伤的影响。方法使用30 mmol/L高糖刺激HUVECs,CCK-8法检测HUVECs活性,免疫荧光法检测HUVECs氧化应激水平,原位末端标记法(TUNEL法)检测HUVECs凋亡水平,免疫荧光法、逆转录聚合酶链反应(PCR)法检测HUVECs PGC-1α蛋白及基因表达水平;逆转录PCR检测超氧化物歧化酶1(Sod1)和Sod2。结果30 mmol/L高糖刺激HUVECs 2 d后其活性明显降低,氧化应激水平明显升高,凋亡水平明显上调,差异均有统计学意义(P<0.05);4-PBA(1.0 mmol/L)干预后可促进PGC-1α高表达,Sod1、Sod2表达明显上调,凋亡水平明显降低,差异均有统计学意义(P<0.05)。结论4-PBA可能通过调节PGC-1α发挥抗氧化效应,抑制高糖诱导的细胞损伤。Objective To investigate the effect of 4-phenyl butyric acid(4-PBA)on the injury of human umbilical vein endothelial cells(HUVECs)induced by high glucose through regulating peroxisome proliferator-activated receptorγcoactivator-1α(PGC-1α).Methods HUVECs were stimulated with 30 mmol/L glucose,and then the CCK-8 assay was used to determine the HUVECs viability.The immunofluorescence method was applied to detect the oxidative stress level of HUVECs.TUNEL was used to detect HUVECs apoptotic level.Immunofluorescence and RT-PCR were performed to detect the protein and gene expression levels of PGC-1α,and RT-PCR was used to detect the Sod1 and Sod2 levels in HUVECs.Results The HUVECs activity was significantly decreased after 30 mmol/L high glucose stimulation for 2 d,the oxidative stress level was significantly increased,the apoptosis level was significantly up-regulated,and the differences were statistically significant(P<0.05).The 4-PBA(1.0 mmol)intervention promoted the high expression of PGC-1α,the Sod1 and Sod2 expression levels were significantly up-regulated,the apoptosis level was significantly decreased,and the differences were statistically significant(P<0.05).Conclusion 4-PBA could play the anti-oxidation effect and inhibit the high glucose induced cellular injury by regulating PGC-1α.

关 键 词：4-苯基丁酸 高糖 人脐静脉内皮细胞 氧化应激 凋亡 过氧化物酶体增殖物激活受体γ共激活因子1α 

分 类 号：R587.2[医药卫生—内分泌]